Basement Membrane Matrix, iPSC-Qualified

Description

Feeder-free culture of human pluripotent stem cells depends on a consistent attachment surface. Variation in the coating matrix can affect colony attachment, morphology and expansion from one passage to the next.

Biofargo Basement Membrane Matrix, iPSC-Qualified is a basement membrane extract prepared from mouse sarcoma tissue and supplied at a protein concentration of 8–12 mg/mL. It is intended for thin-layer coating of culture vessels for feeder-free culture and expansion of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs).

The matrix is liquid at 2–8°C and gels rapidly above 10°C. Its temperature-dependent gelation is reversible, so it can be thawed, aliquoted and diluted on ice before coating.

Specifications

Features

• iPSC-Qualified — Supplied for feeder-free culture and expansion of human iPSCs and ESCs.

• 8–12 mg/mL Protein Concentration — Basement membrane extract suitable for dilution to a thin-layer coating.

• Reversible Temperature-Dependent Gelation — Liquid at 2–8°C and gels above 10°C; prematurely gelled matrix can be returned to a liquid state at 4°C.

• Long Shelf Life — 24 months when stored at −80°C.

• Aliquot-Friendly 5 mL Format — Thaw once, aliquot by intended use, and avoid repeated freeze–thaw cycles.

Instructions for Use

1. Thaw the matrix overnight at 2–8°C. For best temperature control, place the vial on wet ice inside a 4°C refrigerator during thawing.
2. Keep the thawed matrix on ice throughout handling. The matrix gels rapidly above 10°C.
3. Choose the coating thickness and matrix concentration for your application. For feeder-free culture and expansion of hESCs and iPSCs, use a thin-layer coating, not a thick gel.

Handling Notes

• Store at −80°C for long-term storage. Do not use matrix that has been kept at 4°C for more than 24 hours.
• After thawing, aliquot into sterile tubes according to intended use. Avoid repeated freeze–thaw cycles.
• If the matrix gels prematurely, incubate at 4°C for 12–24 hours to restore the liquid state.
• If the matrix looks uneven after thawing, pipette gently up and down until homogeneous.
• To remove air bubbles, centrifuge at 5,000 × g for 1 minute at 0–4°C.
• Follow appropriate laboratory biosafety procedures and wear suitable personal protective equipment.

Applications

• Feeder-free hESC and iPSC culture — Thin-layer coating of plates and flasks for routine pluripotent stem cell maintenance.

• iPSC expansion — Attachment surface for scaling up human iPSC cultures.

• iPSC differentiation workflows — Coated surface for pluripotent stem cells before directed differentiation.

Why Choose Biofargo Basement Membrane Matrix, iPSC-Qualified?

It is supplied specifically for pluripotent stem cell work, with a defined concentration range, a 24-month shelf life and clear handling guidance. It pairs with Biofargo hESC/iPSC expansion media, InstantSelect passaging reagent and PluriCryo cryopreservation solution for a complete feeder-free iPSC workflow.

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