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$365.00
Sku: BFMLIVO100
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by November 20, 2026
Background
Three-dimensional organoid culture has moved from a specialist technique to a standard model system. Primary-tissue organoids retain the cell-type composition, polarity and architecture of the tissue they came from, which two-dimensional hepatocyte culture loses within days — and that gap is why regulators and funders are now pushing non-animal models for toxicology and disease work.
What limits adoption is rarely the biology. It is the medium. Liver organoid establishment from primary tissue depends on a narrow combination of niche factors, and a formulation that is slightly off gives low take rates, loss of hepatic marker expression, or organoids that cannot be passaged. Building that formulation in-house from individual growth factors is expensive and hard to keep consistent between lots.
This kit supplies that formulation as two components. Primary tissue goes in, organoids come out, and the composition is the same from lot to lot.
Description
Primary tissue to organoid. Biofargo Mouse Liver Organoid Medium Kit is a serum-free, defined culture system for the establishment, expansion and long-term maintenance of mouse liver organoids in three-dimensional matrix culture. It is supplied as a basal medium plus a concentrated culture additive, mixed before use.
The kit is designed for the full workflow rather than one step of it: organoid generation from freshly dissociated primary mouse liver tissue, stable maintenance through passaging, and expansion of established lines. The defined, serum-free composition removes serum lot variation as a source of experimental drift, which matters when organoid cultures are carried for weeks.
Under the documented conditions, organoid structures are observed within three days of culture initiation and cultures are suitable for passaging within seven days.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Specifications
| Parameter | Specification |
| Product Name | Biofargo Mouse Liver Organoid Medium Kit |
| Catalog Number | BFMLIVO100 |
| Product Type | Serum-free, defined mouse liver organoid medium kit |
| Starting Material | Primary mouse liver tissue |
| Pack Size | 1 kit (100 mL complete medium) |
| Components | Basal medium 96 mL + culture additive 4 mL |
| Culture Format | 3D culture with extracellular matrix embedding |
| Culture Condition | 37 °C, 5% CO2 |
| Time to Organoid Formation | Structures observed within 3 days; passageable within 7 days |
| Serum | Serum-free |
| Storage | Basal medium 4 °C for 6 months or −20 °C for 12 months; additive −20 °C for 6 months |
| Shipping | Shipped with cold packs |
| Intended Use | For research use only |
Features
Primary tissue to organoid in one kit — establishment, maintenance and expansion are covered by the same medium, so there is no switch between an establishment and a growth formulation partway through a culture.
Serum-free and defined — removes serum lot variation, which is the usual source of drift in organoid cultures carried for weeks.
Organoids within 3 days, passageable at 7 — under the documented conditions, so a tissue harvest reaches a usable culture inside one week.
Two-component format — basal medium plus concentrated additive; mix once and the complete medium is ready, with no multi-factor cocktail to assemble at the bench.
Hepatic marker expression retained — immunofluorescence analysis confirms liver-specific marker expression in organoids cultured in this medium.
Works with standard matrix embedding — compatible with routine basement-membrane matrix workflows and standard plate formats.
Performance Data
Organoid formation and expansion from primary tissue
Following primary liver tissue dissociation, cell collection and matrix embedding, organoid structures were observed during culture progression in Biofargo Mouse Liver Organoid Medium. Early organoid formation occurred within three days, and cultures reached a stage suitable for passaging within seven days.
Hepatic marker expression
Marker analysis of organoids cultured in this medium showed expression patterns associated with liver-derived organoid identity, confirming that hepatic character is retained through culture rather than lost to dedifferentiation.
Applications
Hepatotoxicity and drug safety screening: Liver organoids are the primary non-animal hepatotoxicity model. Establish mouse hepatic organoids for compound response and toxicity studies in a format that retains tissue architecture.
Liver disease modelling: Generate organoids from wild-type, genetically modified or treated animals to study disease mechanism and pathway biology in a tissue-derived model.
Liver biology and development: Study hepatic cell behaviour, tissue organisation and liver-specific processes in three dimensions rather than in monolayer.
Organoid expansion and biobanking: Maintain and expand established mouse liver organoid lines for repeated downstream assays from a single tissue harvest.
Replacement of 2D hepatocyte culture: Primary hepatocytes lose hepatic function within days in monolayer. Organoid culture retains marker expression over passages, supporting longer experimental windows.
Method and protocol development: A defined, consistent medium as the fixed variable when optimising dissociation, embedding or passaging conditions.
Organoid models complement, rather than replace, in vivo work. Findings should be confirmed in the model system appropriate to the research question.
Kit Components
| Component | Volume | Storage |
| Mouse Liver Organoid Basal Medium | 96 mL | 4 °C for 6 months, or −20 °C for 12 months |
| Mouse Liver Organoid Culture Additive | 4 mL | −20 °C for 6 months |
Storage & Shipping
Shipped with cold packs.
Store the basal medium at 4 °C for up to 6 months, or at −20 °C for up to 12 months.
Store the culture additive at −20 °C for up to 6 months.
Mix the additive into the basal medium to prepare complete medium. Aliquot the additive to avoid repeated freeze-thaw cycles.
Prepare only the volume of complete medium needed for near-term use and keep it at 4 °C.
Protocol Overview
Collect mouse liver tissue and wash in PBS.
Dissociate the tissue and prepare liver-derived cells.
Filter and collect cells after digestion.
Embed the cells in basement-membrane matrix for three-dimensional culture.
Prepare complete medium by mixing the culture additive into the basal medium, and overlay the matrix.
Culture at 37 °C, 5% CO2, replacing the complete medium on the documented schedule. Organoid structures appear within 3 days.
Passage within approximately 7 days, when organoids have reached a size suitable for dissociation and re-embedding.
Frequently Asked Questions (FAQs)
Common questions about Biofargo Mouse Liver Organoid Medium Kit.
What is this kit used for?
It is a serum-free, defined medium kit for establishing, maintaining and expanding mouse liver organoids from primary liver tissue in three-dimensional matrix culture.
Does it start from primary tissue or from stem cells?
Primary tissue. Organoids are established from freshly dissociated primary mouse liver. For organoids generated from human pluripotent stem cells, use the iPSC-Derived Liver Organoid Differentiation Kit instead.
How long does organoid formation take?
Under the documented conditions, organoid structures are observed within three days of culture initiation, and cultures are suitable for passaging within seven days. Timing varies with tissue quality, dissociation method and embedding density.
Is the medium serum-free?
Yes. The formulation is serum-free and defined, which removes serum lot variation as a source of experimental drift across long cultures.
What is in the kit, and how is complete medium prepared?
The kit contains 96 mL of basal medium and 4 mL of concentrated culture additive. Mix the additive into the basal medium to give 100 mL of complete medium. Prepare only what is needed in the near term and keep it at 4 °C.
Can the medium support expansion and passaging, not just establishment?
Yes. The same medium covers establishment, long-term maintenance and expansion, so there is no change of formulation partway through a culture.
What matrix and culture conditions are required?
Cells are embedded in basement-membrane matrix and cultured at 37 °C with 5% CO2. The matrix is not included; a growth-factor-reduced, phenol red-free basement membrane matrix is available separately.
Do the organoids retain hepatic identity?
Marker analysis of organoids cultured in this medium shows expression patterns associated with liver-derived organoid identity. This is the practical advantage over two-dimensional primary hepatocyte culture, where hepatic function is lost within days.
How is it stored and shipped?
Shipped with cold packs. Store the basal medium at 4 °C for up to 6 months or at −20 °C for up to 12 months, and the culture additive at −20 °C for up to 6 months.
Can it be used for clinical or diagnostic purposes?
No. This product is for research use only and is not intended for human, diagnostic or therapeutic use.
Related Products
iPSC-Derived Liver Organoid Differentiation Kit — for liver organoids generated from human pluripotent stem cells rather than primary tissue.
Basement Membrane Matrix, Growth Factor Reduced, Phenol Red-Free — matrix for 3D embedding; phenol red-free for fluorescence readouts.
Organoid Tissue Digestion Solution — for dissociating primary tissue before embedding.
OrgCryo Protein-Free Organoid Cryopreservation Solution — for banking established organoid lines.
All organoid culture media — intestinal, lung, gastric, colon, kidney and skin kits in the same format.
Documents
Instruction manual, SDS and lot-specific COA are available on request. Please contact us with the catalog number and lot number.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
