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Specification
| Package | 1 ML/5 ML |
| Full name | Streptactin（Strep-Tag II） Resin 4FF |
| Applications | It can be used for purification of Strep-Tag II labeled proteins in various expression systems, including rod-shaped viruses, mammalian cells, yeast, and bacteria, suitable for most protein properties and facilitating analysis of protein protein protein interactions. At the same time, this resin can also be used for Twin Strep Tag II label purification. |
| Summary | Strep Tag II is a small tag with 8 amino acids (WSHPQFEK), which is only about 1.06 kDa due to its small size and does not affect the structure and function of the fused protein. These mild purification parameters can maintain the biological activity of the protein and yield over 99% purity through only one step of extraction. Among numerous affinity tags, although Strep-tag II appeared later than Flag, c-Myc, and His tags, it has been widely used due to its high affinity, high single-step purification purity (better than His tags), mild purification conditions, and fusion of both ends of the protein. |
| Features | The purification conditions are mild, and the single step purification purity is superior to His label, without affecting the structure and function of the fused protein. It has high affinity and can withstand different buffer conditions and additives (such as high salt, detergent, reducing agent, metal ions, and chelating agent). |
| Composition | 1 mL/5 mL |
| Substrate | Highly cross-linked 4% agarose |
| Average Particle Size | 0 |
| Particle Size | 45 μm~165 μm |
| Protein Binding Ability | ≈4 mg Twin Strep-tag II protein/mL filler |
| Maximum Flow Rate | 300 cm/h |
| Pressurization | 0.3 MPa, 3 bar |
| Storage Buffer | 1X PBS solution containing 20% ethanol |
| Component | 1 mL/5 mL |
Storage
2~8°C, do not freeze
Documents
https://cdn.shopifycdn.net/s/files/1/0521/5312/2997/files/P310130_EN_P.pdf
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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