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Package | 1 KU/5 KU/200 U |
Full name | Taq DNA Polymerase |
Applications | Conventional PCR amplification, RT-PCR, and direct TA cloning of the product. |
Summary | This product was purified from a plasmid cloned with a gene encoding Thermus aquaticus DNA polymerase and induced expression by E. coli. Taq DNA polymerase does not have 3'→5' exonuclease correction activity, but has very low 5'→3' exonuclease activity. Recombinant Taq DNA polymerase is the recommended choice for most PCR reactions, with a half-life of > 40 min at 95°C. The mutation rate of each circulating Taq DNA polymerase < 2.2×10-5. The above-mentioned Taq DNA polymerase is free of contamination by foreign nucleases and bacterial DNA, and has high amplification efficiency and good stability, making it suitable for routine PCR amplification. |
Features | There is no contamination of foreign nuclease and bacterial DNA, and the amplification efficiency is high and the stability is good. |
Composition | Taq DNA polymerase (5 U/ul), 10X PCR Buffer without Mgcl2, 25 mM MgCl2 . |
Reaction Buffer | 10X PCR Buffer |
Unit Definition | One unit is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol deoxyribonucleotides into a polynucleotide fraction in 30 min at 74°C. |
Concentration | 5 U/μl |
Storage Buffer | 20 mM Tris-HCl (PH 8.0) ;0.1 mM EDTA,;1 mM DTT;0.5% Tween 20;100 mM KCl;0.5% NP-40;50% glycerin. |
Component | Taq DNA polymerase (5 U/ul), 10X PCR Buffer without Mgcl2, 25 mM MgCl2 . |
-20°C
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