Hayflick broth (M-HAYFLICK-Y)

Description

HAYFLICK broth is enriched with horse serum, supplying biologically available cholesterol and long-chain fatty acids essential for mycoplasmal membrane biogenesis. Exogenous deoxyribonucleic acid (DNA) is incorporated as a source of purine and pyrimidine bases, which Mycoplasma spp. are unable to synthesize de novo.

Growth-associated acid or alkaline shifts are detected by the phenol red pH indicator, manifesting as a transition of the medium from red to yellow—or occasionally to purple—thus providing a rapid visual proxy for mycoplasma proliferation. Penicillin G is included to selectively suppress concomitant bacterial contaminants while sparing mycoplasmal cells.

Supplementation with a defined lipid mixture (cholesterol and fatty-acid precursors) further enhances biomass yield and colony-forming efficiency, a benefit that is especially pronounced for lipid-dependent strains such as Mycoplasma pneumoniae ATCC 23714.

Specifications

Application

HAYFLICK broth is used for the general detection of mycoplasmas in pharmaceutical products, particularly in bulk vaccines, cell banks, and virus cultures.

Features

• Ready-to-use convenience — no additional preparation steps are required.

• Broad pharmaceutical applications — suitable for biopharmaceuticals, vaccines, cell cultures, and virus cultures.

• Storage stability — can be stored at 2–8 °C for up to 6 months or at −20 °C for 1 year.

• Rapid detection — a colorimetric chart provides a quick and convenient visual indication of early-stage mycoplasma proliferation.

• Optimized growth performance — the buffered system supports higher colony counts while reducing the risk of mycoplasma cell death.

• Enhanced visualization — a chromogenic reagent selectively stains mycoplasma, improving clarity and specificity under microscopy.

• Improved NAD⁺ stability — to prevent growth inhibition caused by NAD⁺ degradation during later stages, alternative NAD⁺ analogs are co-supplemented in the culture system.

Documents

M-HAYFLICK-Y SDS

M-HAYFLICK-Y COA 01

M-HAYFLICK-Y COA 02