Frey broth (M-FREY-Y)

Description

FREY agar is fortified with nicotinamide adenine dinucleotide (NAD⁺) to satisfy the fastidious growth requirements of Mycoplasma synoviae. Metabolic activity of viable mycoplasmas produces acidic by-products that trigger a phenol red pH indicator shift from red to yellow—or, under alkaline drift, to purple—thereby providing an immediate visual confirmation of proliferation. The inclusion of penicillin G selectively suppresses contaminating bacterial flora without impairing mycoplasma growth.

In addition, the medium can be further enriched with a defined lipid supplement from yolk, supplying bioavailable cholesterol and fatty-acid precursors, markedly enhancing colony-forming efficiency—particularly for lipid-dependent strains such as Mycoplasma pneumoniae ATCC 23714.

Specifications

Application

FREY agar is recommended for the detection of avian mycoplasmas in pharmaceutical products, particularly in bulk vaccines, cell banks, and virus cultures.

Features

• Ready-to-use convenience — no additional preparation steps are required.

• Broad pharmaceutical applications — suitable for biopharmaceuticals, vaccines, cell cultures, and virus cultures.

• Storage stability — can be stored at 2–8 °C for up to 3 months or at −20 °C for 6 months.

• Rapid detection — a colorimetric chart provides a quick and convenient visual indication of early-stage mycoplasma proliferation.

• Optimized growth performance — the buffered system supports higher colony counts while reducing the risk of mycoplasma cell death.

• Enhanced visualization — a chromogenic reagent selectively stains mycoplasma, improving clarity and specificity under microscopy.

• Improved NAD⁺ stability — to prevent growth inhibition caused by NAD⁺ degradation during later stages, alternative NAD⁺ analogs are co-supplemented in the culture system.

Documents

M-FREY-Y SDS