Yeast Nitrogen Base w/o Amino Acids (S507)

Description

Yeast Nitrogen Base without Amino Acids contains all essential nutrients and vitamins and inorganic salts necessary for the cultivation of yeasts except histidine, methionine, tryptophane and a source of carbohydrate. 

Use:

Yeast Nitrogen Base without Amino Acids is used for classifying yeasts based on 
amino acid and carbohydrate requirements. 

Formula (per Liter)

Final pH: 5.4 ± 0.2 at 25.0°C 
* Grams per liter may be adj. or formula supplemented to obtain desired performance 

Preparation

Prepare a 10X solution by dissolving 6.7 grams of Yeast Nitrogen Base without Amino Acids, 5 grams of Dextrose or equivalent amount of other carbohydrate and 5-10 mg of the desired amino acid in 100ml distilled or deionized water. Warm slightly to dissolve. Mix well. DO NOT BRING TO A BOIL. DO NOT AUTOCLAVE. Filter-sterilize the solution. Store at 2-8°C. Prepare the final medium by aseptically pipetting 0.5ml of the 10X solution into 4.5ml of sterile distilled water. Mix the solution thoroughly by shaking before inoculation.

Quality Control Specifications

• The powder is homogeneous, free flowing and off-white. 
• Visually the prepared medium is clear and almost colorless. 
• Expected cultural response after 2-5 days at 25.0°C. 

Storage

Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original off-white color.

Documents

INFO

SDS