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Description
Yeast Nitrogen Base without Amino Acids and Ammonium Sulfate contains all essential nutrients and vitamins necessary for the cultivation of yeasts except amino acids and a source of nitrogen and carbohydrate.
Use
Yeast Nitrogen Base without Amino Acids and Ammonium Sulfate is used for classifying yeasts based on carbon and nitrogen requirements.
Formula (per Liter)
| Biotin | 0.002mg | Calcium Pantothenate | 0.4mg |
| Folic Acid | 0.002mg | Inositol | Inositol |
| Niacin | 0.4mg | p-Aminobenzoic Acid | 0.2mg |
| Pyridoxine Hyrochloride | 0.4mg | Riboflavin | 0.2mg |
| Thiamine Hydrochloride | 0.4mg | Boric Acid | 0.5mg |
| Copper Sulfate | 0.04mg | Potassium Iodide | 0.1mg |
| Ferric Chloride | 0.2mg | Manganese Sulfate | 0.4mg |
| Sodium Molybdate | 0.2mg | Zinc Sulfate | 0.4mg |
| Monopotassium Phosphate | 1.0g | Magnesium Sulfate | 0.5g |
| Sodium Chloride | 0.1g | Calcium Chloride | 0.1g |
Final pH: 4.5 ± 0.2 at 25.0°C
* Grams per liter may be adj. or formula supplemented to obtain desired performance
Preparation
Prepare a 10X solution by dissolving 1.7 grams of Yeast Nitrogen Base without Amino Acids and Ammonium. Sulfate and nitrogen and carbon sources, as required, in 100ml distilled or deionized water. Warm slightly to dissolve. Mix well. DO NOT BRING TO A BOIL. DO NOT AUTOCLAVE. Filter-sterilize the solution. Store at 2-8°C. Prepare the final medium by aseptically pipetting 0.5ml of the 10X solution into 4.5ml of sterile distilled water. Mix the solution thoroughly by shaking before inoculation.
Quality Control Specifications
- The powder is homogeneous, free flowing and off-white.
- Visually the prepared medium is clear and almost colorless.
- Expected cultural response after 2-5 days at 25.0°C.
| Organism | Result with Additives |
| Candida albicans ATCC 10231 | Good Growth |
| Kloeckera apiculata ATCC 9774 | Good Growth |
| Saccharomyces pastorianus ATCC 9080 | Good Growth |
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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