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Rapid and Sensitive Detection of Yellow Fever Vaccine Strain 17D – Suitable for Clinical and Research Use, 50 Reactions
$641.00
Sku: 15-14310
Categories:
Cell & Molecular Biology
, Custom Solutions
, Pathogen Detection Kits
, qPCR Kit
Description
Yellow Fever Virus Vaccine Strain 17D Probe qRT-PCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.
Key Features
One-tube qPCR format minimizes carry-over contamination risk
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High sensitivity: analytical LOD as low as 100 copies/reaction
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Excellent specificity: no cross-reactivity with DNA from other pathogens
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Includes amplification positive control for result verification
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Instrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc.
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Suitable for both qualitative and quantitative applications
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For quantitative use: the linear range covers a 100,000-fold concentration difference.
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Sufficient for 50 reactions (20 μL each)
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For Research Use Only (RUO)
Kit Components
| Component Name | Product Number. | Size | Vial | Lid Color |
| 2×Probe qPCR MasterMix | 981201 | 0.5 mL | 0.5 mL Vial | Natural |
| Template Dilution Buffer | 180701 | 1 mL | 1.5 mL Vial | Green |
| Primer-Probe Powder | yp15-14310 | 50 T | 0.5 mL Brown Vial | Brown |
| Amplification Positive Control (1E7 copies/μL) |
pc14310 | 50 μL | 0.5 mL Vial | Yellow |
| Ultrapure water | 210806 | 1 mL | 1.5 mL Vial | Blue |
| Manual | sc15-14310 | 1 Set | NA | NA |
Application Areas
- Aquaculture Health Management – Rapid qRT-PCR screening of Yellow Fever Virus Strain 17D for early disease detection and outbreak prevention in abalone, shrimp and other marine farming operations.
- Marine Pathogen Surveillance & Ecological Risk Assessment – Sensitive monitoring of 17D contamination in seawater, sediment and marine biota to support regulatory compliance and ecosystem health studies.
- Vaccine Manufacturing Quality Assurance – Quantitative control of Yellow Fever Virus vaccine seed strain 17D in in-process samples and final product release assays for GMP-driven production workflows.
Storage & Shipping
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Store at - 20 °C
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The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.
Documents
Yellow Fever Virus Vaccine Strain 17D Probe qRT-PCR Kit User Manual
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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