XR NKCryo - Serum-Free NK Cell Cryopreservation Solution

Features

• NK Cell-Specific Cryopreservation Solution — XR NKCryo is designed specifically for human natural killer cell cryopreservation workflows, helping laboratories maintain NK cell quality during freezing, transportation and thawing procedures.

• Serum-Free and Animal Component-Free Formulation — The formulation contains no human- or animal-derived components, reducing the risk of introducing undefined biological materials during NK cell storage.

• Protein-Free Cryopreservation Medium — XR NKCryo contains no protein components, supporting applications requiring controlled cryopreservation conditions for immune cell research.

• Optimized for Human NK Cell Freezing — Suitable for NK cells generated from peripheral blood mononuclear cells (PBMCs) and cord blood mononuclear cell sources.

• High-Density NK Cell Freezing Capability — Supports cryopreservation densities ranging from 1 × 10⁶ to 5 × 10⁸ cells/mL, accommodating both routine laboratory storage and high-density immune cell banking workflows.

• Supports Post-Thaw NK Cell Recovery — Designed to minimize freezing-related stress and maintain high post-thaw cell viability and recovery after cryopreservation.

• Low Endotoxin Cell Culture Grade — Manufactured with cell culture-grade raw materials and controlled endotoxin levels for reliable immune cell research applications.

• Compatible with NK Cell Expansion Workflows — Can be integrated into workflows involving NK cell activation, expansion, freezing and subsequent recovery culture.

Performance Data

Post-Thaw NK Cell Viability After Cryopreservation

Cryopreservation performance was evaluated using human NK cells with a CD3^-CD56⁺ proportion greater than 90%. Cells were generated from cord blood-derived mononuclear cells and expanded using an XR NK activation workflow before freezing with XR NKCryo.

The evaluation demonstrated that XR NKCryo supports high NK cell survival after cryopreservation. Under high-density freezing conditions, cells maintained post-thaw viability above 90% after 3 months of storage and maintained viability above 80% during a 6-month storage period.

Post-thaw viability analysis of human NK cells preserved with XR NKCryo NK cell cryopreservation medium

NK Cell Recovery After Long-Term Storage

Cell recovery analysis demonstrated that XR NKCryo maintains a high recovery rate following extended cryopreservation. After 6 months of storage, frozen NK cells maintained more than 80% cell recovery after thawing.

Human NK cell recovery rate after long-term storage using serum-free NK cell cryopreservation medium

Recommended Cryopreservation Workflow

XR NKCryo provides a standardized workflow for laboratories performing human NK cell expansion, freezing and recovery experiments. The recommended process includes cell harvesting, cryoprotectant addition, controlled-rate freezing and transfer to long-term storage.

Step 1: NK Cell Collection

After completion of NK cell culture and expansion, collect NK cells by centrifugation and carefully remove the culture supernatant.

Step 2: Add XR NKCryo Freezing Medium

Slowly add XR NKCryo cryopreservation medium to the cell pellet. Gently resuspend the cells to achieve a uniform suspension.

Recommended final freezing density:

• 1 × 10⁶ to 5 × 10⁸ cells/mL

Step 3: Controlled Cooling and Storage

Transfer prepared cryovials into a controlled-rate freezing container and place them at -80°C. For long-term storage, transfer cells to liquid nitrogen storage after approximately 12 hours.

Step 4: NK Cell Thawing and Recovery

Thaw frozen NK cells using a 37°C water bath or cell recovery instrument. Transfer cells into a centrifuge tube and add at least three volumes of serum-free NK cell culture medium or physiological saline to dilute the cryopreservation solution. Centrifuge to remove the freezing medium before downstream experiments.

Applications

XR NKCryo Serum-Free NK Cell Cryopreservation Medium is designed for laboratories working with human natural killer cells, immune cell expansion platforms and cell-based research workflows requiring reliable frozen cell preservation.

The product supports applications where maintaining NK cell viability, recovery and consistency after thawing is important, including immune cell research, NK cell banking and cell therapy development workflows.

• Human NK Cell Cryopreservation — Supports freezing and storage of expanded human NK cells while maintaining post-thaw viability for further research.

• NK Cell Expansion and Cryopreservation — Suitable for workflows combining NK cell activation, expansion, harvesting and long-term frozen storage.

• PBMC-Derived NK Cell Preservation — Designed for NK cells generated from peripheral blood mononuclear cell sources.

• Cord Blood NK Cell Cryopreservation — Applicable for research workflows using cord blood-derived NK cell populations.

• Immune Cell Cryopreservation Research — Provides a specialized cryopreservation solution for immune cell-based laboratory studies.

• CAR-NK Cell Research — Suitable for research workflows where expanded NK cell populations require freezing, storage and recovery before functional analysis.

• NK Cell Banking — Supports establishment of frozen NK cell stocks for repeated experimental use and longitudinal studies.

• Post-Thaw NK Cell Functional Studies — Enables recovery of NK cells for downstream assays including phenotype analysis, expansion studies and immune function evaluation.

Advantages for NK Cell Research Workflows

Maintaining consistent NK cell quality after freezing is an important consideration in immune cell research. Variations in freezing conditions, cryoprotectant formulations and cell density may influence recovery performance and experimental reproducibility.

XR NKCryo provides a dedicated NK cell freezing solution designed around the requirements of human NK cell preservation. Its serum-free, protein-free and animal component-free formulation helps researchers establish controlled cryopreservation workflows while supporting high-density NK cell storage.

By combining optimized cryoprotection, low endotoxin manufacturing control and compatibility with NK expansion workflows, XR NKCryo helps laboratories reduce variability between fresh and frozen NK cell experiments.

Experimental Considerations

• Use freshly harvested and healthy NK cells with appropriate viability before cryopreservation.

• Maintain recommended freezing density to achieve consistent cryopreservation performance.

• Use controlled-rate freezing procedures whenever possible to reduce freezing stress.

• Avoid repeated freeze-thaw cycles of cryopreserved NK cell samples.

• Evaluate post-thaw viability and recovery before downstream functional assays.