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Sku: XREpiGM-M-500
XR Human Epithelial Cell Medium Kit
Description
Primary epithelial cells can be difficult to establish and expand because limited attachment, slow spreading, early senescence and loss of epithelial characteristics may reduce experimental consistency. A general-purpose basal medium may not provide the tissue-adapted nutrients and growth support required by sensitive primary epithelial cell cultures.
XR Epithelial Cell Growth Medium Kit is a low-serum primary epithelial cell culture medium developed for epithelial cells derived from respiratory, ocular, renal, mammary, prostate and other tissues. Its optimized basal formulation supports cell attachment and spreading, while the selected epithelial growth supplement provides balanced nutritional support for cell survival and proliferation.
The complete culture system contains recombinant human epidermal growth factor and transferrin together with 2% v/v fetal bovine serum. This low-serum formulation is designed to reduce excessive serum exposure while providing the growth support needed to help delay primary epithelial cell senescence and maintain epithelial phenotype and key cellular functions.
Two tissue-oriented supplement options are available. EpiGM-B is intended for retinal pigment epithelial cell culture, while EpiGM-M supports a broader range of primary epithelial cell types, including bronchial, alveolar, mammary, corneal, conjunctival, renal, renal proximal tubule, sebaceous gland and prostate epithelial cells.
Important: XR Epithelial Cell Growth Medium is a low-serum medium containing 2% v/v fetal bovine serum. It is not a serum-free epithelial cell culture medium.
Specifications
| Product Name | XR Epithelial Cell Growth Medium Kit |
| Product Type | Low-serum primary epithelial cell growth medium kit |
| Kit Reference | XREpiGM-500-A |
| Basal Medium | Epi Basal Medium, 480 mL |
| Supplement Options | EpiGM-M Supplement, 20 mL EpiGM-B Supplement, 20 mL |
| Supplement References | XREpiGM-500-(M)B XREpiGM-500-(B)B |
| Serum Content | 2% v/v fetal bovine serum in the complete formulation |
| Growth Components | Recombinant human epidermal growth factor and transferrin |
| pH | 7.2–7.4 |
| Endotoxin | ≤0.1 EU/mL |
| Filtration | <0.2 μm |
| Bacteria Detection | Not detected |
| Fungi Detection | Not detected |
| Antibiotics | Not included |
| Antifungal Agents | Not included |
| Basal Medium Storage | 2–8°C, protected from light |
| Supplement Storage | -20°C, protected from light |
| Prepared Medium Storage | 2–8°C for up to 4 weeks |
| Shelf Life | 12 months |
| Research Grade | For Research Use Only |
Medium Selection Guide
Select the supplement according to the epithelial cell type being cultured. The basal medium is combined with either EpiGM-B or EpiGM-M to create the appropriate complete primary epithelial cell growth medium.
| Complete Medium Configuration | Recommended Cell Types |
|---|---|
| Epi Basal Medium + EpiGM-B | Retinal pigment epithelial cells |
| Epi Basal Medium + EpiGM-M | Bronchial epithelial cells, alveolar epithelial cells, mammary epithelial cells, corneal epithelial cells, conjunctival epithelial cells, renal epithelial cells, renal proximal tubule epithelial cells, sebaceous gland cells and prostate epithelial cells |
Features
- Low-serum epithelial cell culture medium containing 2% v/v fetal bovine serum
- Designed for primary epithelial cell attachment, spreading, survival and expansion
- Contains selected epithelial cell nutrients for balanced culture support
- Contains recombinant human epidermal growth factor to support epithelial cell proliferation
- Contains transferrin to support iron transport and cellular metabolism
- Helps reduce premature senescence during primary epithelial cell culture
- Designed to maintain epithelial cell phenotype and key cellular functions
- Two supplement options for retinal pigment epithelial cells and other tissue-derived epithelial cells
- Low endotoxin specification of ≤0.1 EU/mL
- Filtered through a <0.2 μm filtration process
- Supplied without antibiotics or antifungal agents for flexible experimental control
- Convenient basal medium and supplement format for complete or partial preparation
Culture Medium Preparation
Complete 500 mL Preparation
Thaw the appropriate EpiGM supplement at 4°C overnight or at room temperature for 1–2 hours. Add the supplement to the Epi basal medium and mix thoroughly to prepare the complete epithelial cell culture medium.
Partial Preparation
For experiments that do not require the entire kit at one time, aliquot the supplement and prepare the medium using the following ratio:
| EpiGM Supplement | 4 mL |
| Epi Basal Medium | 96 mL |
| Final Volume | 100 mL |
Store the prepared complete medium at 2–8°C and use within four weeks.
Optional Antibiotic Addition
The product does not contain antibiotics or antifungal agents. When required by the experimental protocol, an antibiotic solution may be added at 0.5–1% of the final culture volume.
Recommended Culture Conditions
| Culture Stage | Recommended Seeding Density | Medium Change | Recommended Passage Point |
|---|---|---|---|
| Rapid Cell Growth | 10,000–15,000 cells/cm² | Change medium every other day | 70–80% confluence |
| Routine Passage | 5,000–8,000 cells/cm² | Change medium every other day | 70–80% confluence |
The optimal seeding density may vary according to cell source, donor, passage number, culture vessel, coating system and experimental objective. Researchers should confirm the appropriate conditions for their specific primary epithelial cell model.
Applications
XR Epithelial Cell Growth Medium Kit supports research workflows that require controlled culture and expansion of primary epithelial cells from multiple tissue sources. Its low-serum formulation and tissue-oriented supplement options make it suitable for epithelial cell isolation, routine propagation, morphology monitoring and downstream functional studies.
Primary Epithelial Cell Culture and Expansion
- Establishment of primary epithelial cell cultures from tissue-derived samples
- Primary human epithelial cell attachment and spreading studies
- Routine propagation and expansion of epithelial cell populations
- Evaluation of epithelial cell morphology during in vitro culture
- Preparation of epithelial cells for downstream molecular and cellular assays
Airway and Pulmonary Epithelial Cell Culture
- Bronchial epithelial cell culture and expansion
- Airway epithelial cell growth studies
- Alveolar epithelial cell culture research
- Respiratory epithelial cell morphology and proliferation analysis
- In vitro studies involving respiratory tissue-derived epithelial cells
Ocular Epithelial Cell Research
- Retinal pigment epithelial cell culture using EpiGM-B supplement
- RPE cell attachment, maintenance and expansion studies
- Corneal epithelial cell culture using EpiGM-M supplement
- Conjunctival epithelial cell growth and morphology studies
- Research involving ocular surface and retinal epithelial cell models
Renal Epithelial Cell Culture
- Renal epithelial cell culture and proliferation research
- Kidney epithelial cell maintenance in vitro
- Renal proximal tubule epithelial cell culture
- Evaluation of renal epithelial cell morphology and growth behavior
- Preparation of kidney-derived epithelial cells for downstream assays
Mammary and Prostate Epithelial Cell Research
- Primary mammary epithelial cell culture and expansion
- Mammary epithelial cell proliferation studies
- Primary prostate epithelial cell culture
- Prostate epithelial cell morphology and phenotype research
- In vitro studies involving glandular epithelial cell populations
Sebaceous Gland Cell Culture
- Culture and expansion of sebaceous gland-derived cells
- Evaluation of sebaceous cell morphology and proliferation
- Preparation of sebaceous gland cells for cellular research workflows
Epithelial Cell Phenotype and Function Studies
- Evaluation of epithelial morphology during primary cell expansion
- Studies of cell attachment and spreading
- Assessment of epithelial cell proliferation and survival
- Research involving maintenance of epithelial characteristics in vitro
- Comparison of culture conditions for tissue-derived epithelial cells
Why Choose XR Epithelial Cell Growth Medium Kit?
Successful primary epithelial cell culture requires more than basic nutrient supplementation. Cells isolated from tissues may attach inefficiently, expand slowly or undergo early senescence when culture conditions are not adapted to epithelial cell requirements.
XR Epithelial Cell Growth Medium Kit combines an optimized basal medium with selected epithelial supplements, recombinant human epidermal growth factor, transferrin and a controlled 2% serum concentration. This formulation is designed to provide sufficient growth support without relying on a conventional high-serum culture system.
The availability of separate EpiGM-B and EpiGM-M supplements allows researchers to select a culture configuration according to the epithelial cell source. This makes the system suitable for retinal pigment epithelial cells as well as respiratory, ocular surface, renal, mammary, prostate and sebaceous gland-derived epithelial cells.
- Designed specifically for primary epithelial cell culture rather than general-purpose cell maintenance
- Supports cell attachment, spreading, survival and proliferation
- Low-serum formulation containing 2% v/v fetal bovine serum
- Contains recombinant human EGF and transferrin
- Supports multiple tissue-derived epithelial cell types
- Includes separate supplement options for RPE cells and other epithelial cells
- Low endotoxin and <0.2 μm filtered formulation
- Supplied without antibiotics or antifungal agents
- Suitable for complete-bottle or partial-volume preparation
FAQ
What is XR Epithelial Cell Growth Medium Kit used for?
XR Epithelial Cell Growth Medium Kit is used for the in vitro culture and expansion of primary epithelial cells. It is designed to support cell attachment, spreading, survival, proliferation and epithelial phenotype maintenance.
What is primary epithelial cell growth medium?
Primary epithelial cell growth medium is a specialized culture formulation that provides nutrients and growth-supporting components for epithelial cells isolated from tissues. Unlike a general-purpose medium, it is designed around the attachment, proliferation and phenotype requirements of primary epithelial cells.
Is XR Epithelial Cell Growth Medium serum-free?
No. This product is a low-serum epithelial cell culture medium containing 2% v/v fetal bovine serum. It should not be marketed or described as serum-free.
Which supplement should be used for retinal pigment epithelial cells?
Retinal pigment epithelial cells should be cultured using Epi basal medium combined with EpiGM-B supplement.
Which supplement should be used for bronchial or alveolar epithelial cells?
Bronchial and alveolar epithelial cells should be cultured using Epi basal medium combined with EpiGM-M supplement.
Can the medium be used for corneal epithelial cell culture?
Yes. Epi basal medium combined with EpiGM-M supplement is intended for corneal epithelial cell culture. The same configuration can also be used for conjunctival epithelial cells.
Can the medium be used for renal proximal tubule epithelial cells?
Yes. The Epi basal medium and EpiGM-M supplement configuration is suitable for renal epithelial cells and renal proximal tubule epithelial cells.
Can the medium be used for mammary and prostate epithelial cells?
Yes. EpiGM-M complete medium is intended for primary mammary epithelial cells and prostate epithelial cells, as well as several other tissue-derived epithelial cell types.
Does the medium contain epidermal growth factor?
Yes. The formulation contains recombinant human epidermal growth factor to support epithelial cell proliferation and culture performance.
Does the medium contain transferrin?
Yes. Transferrin is included as part of the epithelial cell growth-supporting formulation.
Does XR Epithelial Cell Growth Medium contain antibiotics?
No. The supplied product does not contain antibiotics or antifungal agents. Laboratories may add an antibiotic solution at 0.5–1% when required by their protocol.
How should the EpiGM supplement be thawed?
Thaw the supplement at 4°C overnight or at room temperature for approximately 1–2 hours before mixing it with the basal medium.
What is the recommended mixing ratio for partial medium preparation?
For partial preparation, combine 4 mL of EpiGM supplement with 96 mL of Epi basal medium to prepare 100 mL of complete epithelial cell growth medium.
How long can the prepared complete medium be stored?
The prepared complete medium can be stored at 2–8°C for up to four weeks.
What seeding density is recommended for rapid epithelial cell growth?
For rapid growth, a seeding density of approximately 10,000–15,000 cells/cm² is recommended. Change the medium every other day and passage the cells at approximately 70–80% confluence.
What seeding density is recommended for routine passage?
For routine passage, a seeding density of approximately 5,000–8,000 cells/cm² is recommended. Change the medium every other day until the culture reaches approximately 70–80% confluence.
What are the storage conditions for the kit components?
Store the Epi basal medium at 2–8°C protected from light. Store EpiGM-M and EpiGM-B supplements at -20°C protected from light.
What is the endotoxin specification?
The endotoxin specification is ≤0.1 EU/mL.
Is this product intended for clinical use?
No. XR Epithelial Cell Growth Medium Kit is intended for research use only and is not intended for diagnostic, therapeutic or clinical applications.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
