XR hES/iPSC Adherent Culture Medium Kit (Low-protein, AOF)

Description

Maintaining consistent expansion and pluripotent characteristics of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) can be challenging during routine stem cell culture workflows. Variations in cell state, passage number, recovery after thawing, and culture conditions may affect proliferation performance and downstream pluripotency analysis.

XR hES/iPSC Adherent Culture Medium Kit (Low-protein, AOF) is a defined human pluripotent stem cell culture medium developed for feeder-free maintenance and expansion of human ES cells and induced pluripotent stem cells. The low-protein formulation and animal-origin-free (AOF) design help reduce complex protein background and provide a more controlled culture environment for iPSC research workflows.

Designed for researchers requiring consistent human iPSC culture conditions, XR hES/iPSC Medium supports adherent stem cell expansion while maintaining commonly evaluated pluripotency characteristics. The formulation is suitable for routine iPSC maintenance, expansion studies, and stem cell research applications requiring a defined and controlled culture system.

Unlike traditional culture systems containing undefined biological components, this low-protein iPSC culture medium provides an animal-origin-free formulation designed to improve experimental consistency and reduce variability associated with complex protein backgrounds.

Specifications

Features

• Feeder-free culture medium designed for human ES and iPS cell maintenance and expansion
• Low-protein formulation helps reduce complex protein background during stem cell culture
• Animal-Origin-Free (AOF) formulation without animal-derived or human-derived components
• Defined culture environment supporting controlled iPSC research workflows
• Suitable for adherent human pluripotent stem cell culture systems
• Supports expansion and maintenance of hESC and iPSC populations
• Filtered through a <0.2 μm filtration process for culture preparation
• Compatible with pluripotency evaluation workflows including SSEA-3 and TRA-1-81 analysis

Performance Data

Human iPSC Expansion Performance Comparison

XR hES/iPSC Low-protein Culture Medium was evaluated for human induced pluripotent stem cell expansion performance compared with TeSR™-E8™ medium. Three different iPSC sources were cultured under adherent conditions for five passages, and viable cell numbers were analyzed using AO/PI staining.

The evaluation showed that XR low-protein iPSC culture medium achieved higher average expansion performance compared with TeSR™-E8™ under the tested experimental conditions.

Expansion Performance Across Multiple iPSC Lines

Three independent iPSC cell sources were evaluated to compare average expansion fold after continuous culture. XR hES/iPSC Medium demonstrated consistent expansion performance across tested cell lines.

Cell Viability Analysis

Cell viability was analyzed during five-passage culture experiments. Results showed no significant difference in average viability between XR low-protein medium and TeSR™-E8™ under the tested conditions.

Maintenance of Human Pluripotency Markers

Maintaining pluripotent characteristics is an important consideration during human embryonic stem cell (hESC) and induced pluripotent stem cell (iPSC) culture. XR hES/iPSC Adherent Culture Medium Kit was evaluated using commonly used pluripotency markers including SSEA-3 and TRA-1-81 during continuous culture experiments.

SSEA-3 Expression Analysis

SSEA-3 expression levels were evaluated after continuous passage culture. The results showed comparable SSEA-3 expression between XR low-protein iPSC culture medium and TeSR™-E8™ under the tested experimental conditions.

TRA-1-81 Expression Analysis

TRA-1-81 expression was analyzed as an additional pluripotency-associated marker. XR low-protein hPSC culture medium demonstrated higher TRA-1-81 expression levels compared with TeSR™-E8™ in the reported evaluation.

Applications

XR hES/iPSC Adherent Culture Medium Kit (Low-protein, AOF) is designed for researchers performing human pluripotent stem cell culture workflows requiring defined formulation, reliable expansion performance, and maintenance of iPSC characteristics.

• Human induced pluripotent stem cell (iPSC) maintenance and expansion
• Human embryonic stem cell (hESC) adherent culture
• Feeder-free human pluripotent stem cell culture workflows
• Expansion of established iPSC cell lines
• Maintenance of high-passage human iPSC cultures
• Recovery and expansion studies after iPSC thawing
• Stem cell characterization studies using pluripotency markers
• Human iPSC disease modeling research
• Stem cell differentiation research requiring expanded pluripotent populations
• Basic stem cell biology research and culture method development

Why Choose XR Low-Protein AOF iPSC Culture Medium?

Human pluripotent stem cell culture requires stable and reproducible conditions to support consistent cell expansion and downstream characterization. Variations caused by complex biological components may introduce additional factors during routine iPSC culture workflows.

XR hES/iPSC Adherent Culture Medium Kit provides a low-protein, animal-origin-free formulation designed for researchers seeking a controlled human pluripotent stem cell culture environment. The defined formulation supports feeder-free iPSC maintenance and expansion while enabling evaluation of commonly used pluripotency markers.

• Low-protein formulation helps minimize complex protein background
• Animal-Origin-Free (AOF) formulation supports defined culture workflows
• Designed for human pluripotent stem cell expansion and maintenance
• Supports iPSC characterization workflows including SSEA-3 and TRA-1-81 analysis

FAQ

What is XR hES/iPSC Adherent Culture Medium Kit used for?

XR hES/iPSC Adherent Culture Medium Kit is a human pluripotent stem cell culture medium designed for feeder-free maintenance and expansion of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs).

What makes XR low-protein iPSC culture medium different from conventional stem cell media?

XR iPSC culture medium features a low-protein, animal-origin-free (AOF) formulation designed to reduce complex protein background and provide a more controlled culture environment for human pluripotent stem cell research.

Is XR hES/iPSC Medium suitable for feeder-free iPSC culture?

Yes. XR hES/iPSC Adherent Culture Medium Kit is designed for feeder-free adherent human ES and iPSC culture workflows.

What cells can be cultured using XR hES/iPSC Medium?

XR hES/iPSC Medium is designed for human embryonic stem cells (hESCs) and human induced pluripotent stem cells (iPSCs) maintained under adherent culture conditions.

What does AOF mean in XR iPSC culture medium?

AOF means Animal-Origin-Free. The formulation is designed without animal-derived or human-derived components, helping reduce variability associated with complex biological components.

Does XR low-protein iPSC medium support pluripotency marker analysis?

Yes. XR hES/iPSC Medium has been evaluated using commonly used pluripotency markers including SSEA-3 and TRA-1-81 during continuous culture experiments.

How was XR hES/iPSC Medium evaluated for expansion performance?

XR hES/iPSC Medium was evaluated through five-passage human iPSC culture experiments. Expansion performance was analyzed using AO/PI staining-based viable cell counting, and pluripotency markers were evaluated under the reported conditions.

What is the storage condition for XR hES/iPSC Culture Medium Kit?

The basal medium should be stored at 2–8°C, while the additive component should be stored at -20°C.

What is included in XRiPSC-E-500?

XRiPSC-E-500 contains 496 mL basal medium and 4 mL additive solution.