XR hES/iPSC Adherent Culture Medium Kit (High-protein, AOF)

Description

Maintaining reliable expansion of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) requires a culture environment that supports cell proliferation while preserving key pluripotency characteristics. During routine stem cell culture workflows, variations in cell recovery after thawing, passage adaptation, and expansion efficiency may affect the availability of high-quality pluripotent cell populations for downstream research.

XR hES/iPSC Adherent Culture Medium Kit (High-protein, AOF) is a human pluripotent stem cell culture medium designed for feeder-free adherent culture of human ES cells and iPS cells. The formulation contains recombinant human serum albumin and provides a defined culture environment without animal-origin components or human-origin components, supporting consistent stem cell maintenance and expansion workflows.

This AOF iPSC culture medium is developed for researchers requiring reliable human pluripotent stem cell expansion while maintaining commonly evaluated pluripotency markers. Performance evaluation demonstrated enhanced cell expansion compared with TeSR™-AOF under tested conditions, while maintaining comparable cell viability and pluripotency marker expression including SSEA-3 and TRA-1-81.

Specifications

Features

• Animal-origin-free (AOF) formulation designed for human pluripotent stem cell culture workflows without animal-origin components.

• High-protein culture formulation containing recombinant human serum albumin to support iPSC proliferation and culture performance.

• Designed for feeder-free iPSC culture and adherent maintenance of human ES and iPS cells.

• Supports human pluripotent stem cell expansion with enhanced cell recovery and proliferation performance.

• Compatible with evaluation of commonly used pluripotency markers including SSEA-3 and TRA-1-81.

• Provides a defined culture system for researchers performing hESC and iPSC maintenance studies.

• Manufactured with filtration and quality control testing including endotoxin, bacteria, and fungi detection.

Performance Data

Enhanced iPSC Expansion Performance Compared with TeSR™-AOF

XR hES/iPSC High-protein AOF Medium was evaluated against TeSR™-AOF using continuous five-passage human iPSC culture experiments. High-passage iPSC cells were recovered and expanded using adherent culture conditions on Corning Matrigel-coated plates. Cell expansion performance was analyzed by AO/PI staining-based viable cell counting.

The evaluation showed that XR high-protein AOF medium produced higher cumulative viable cell numbers compared with TeSR™-AOF under tested culture conditions.

Expansion Performance Across Different iPSC Sources

Three different human iPSC cell sources were evaluated using XR high-protein AOF medium and TeSR™-AOF. Average expansion performance across five passages was analyzed to assess consistency among different iPSC backgrounds.

XR hES/iPSC Medium demonstrated higher average expansion performance across tested iPSC sources while maintaining comparable culture characteristics.

Cell Viability Evaluation During iPSC Expansion

AO/PI staining analysis showed comparable cell viability between XR high-protein AOF medium and TeSR™-AOF during the evaluation period.

Pluripotency Marker Maintenance

Maintaining pluripotency characteristics is an important consideration during human pluripotent stem cell culture. XR hES/iPSC Medium was evaluated using SSEA-3 and TRA-1-81 expression analysis.

SSEA-3 expression levels showed no significant difference between XR high-protein AOF medium and TeSR™-AOF under tested conditions.

TRA-1-81 expression analysis demonstrated comparable pluripotency marker maintenance between tested culture conditions.

Applications

XR hES/iPSC Adherent Culture Medium Kit (High-protein, AOF) is designed for researchers performing human pluripotent stem cell culture workflows that require consistent maintenance and expansion of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). The animal-origin-free formulation and high-protein culture environment support routine feeder-free adherent stem cell culture applications.

• Human induced pluripotent stem cell (iPSC) expansion
  Supports expansion workflows where researchers require increased recovery and proliferation performance during repeated iPSC culture passages.

• Human embryonic stem cell (hESC) culture
  Suitable for adherent culture maintenance of human embryonic stem cells under defined culture conditions.

• Feeder-free iPSC culture workflows
  Designed for feeder-free human pluripotent stem cell culture systems requiring reliable maintenance and expansion.

• Human pluripotent stem cell maintenance
  Supports routine hPSC culture workflows while maintaining commonly evaluated pluripotency characteristics.

• High-passage iPSC expansion studies
  Suitable for researchers evaluating expansion performance after multiple culture passages.

• iPSC recovery after thawing
  Provides a culture environment for recovery and subsequent expansion of thawed human iPSC populations.

• Stem cell characterization studies
  Compatible with workflows evaluating pluripotency markers including SSEA-3 and TRA-1-81.

• Human iPSC disease modeling research
  Supports preparation and expansion of human iPSC populations used in stem cell research models.

• Stem cell differentiation research preparation
  Provides expanded pluripotent stem cell populations for downstream differentiation research workflows.

• Basic human pluripotent stem cell biology research
  Suitable for laboratory studies requiring consistent hESC/iPSC culture conditions.

Why Choose XR hES/iPSC Culture Medium?

Human pluripotent stem cell culture requires a reliable environment that supports cell expansion while preserving important characteristics associated with pluripotency. Differences in cell source, passage number, and recovery conditions may influence expansion performance during routine hESC and iPSC culture workflows.

XR hES/iPSC Adherent Culture Medium Kit combines an animal-origin-free (AOF) formulation with recombinant human serum albumin to provide a defined culture solution for feeder-free human pluripotent stem cell expansion. The formulation is designed to support researchers looking for a consistent iPSC culture medium for adherent hESC and iPSC maintenance.

In comparative evaluation against TeSR™-AOF, XR high-protein AOF medium demonstrated increased cumulative viable cell numbers during five-passage iPSC expansion experiments while maintaining comparable cell viability and pluripotency marker expression.

FAQ