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Efficient, Low-Toxicity Transfection for DNA and RNA Delivery in Eukaryotic Cells
$550.00
Sku: TR001
Categories:
Description
Transfection Reagent is a next-generation cationic lipid-based delivery system optimized for efficient transfection of plasmid DNA, messenger RNA (mRNA), and small interfering RNA (siRNA) into a broad range of eukaryotic cells. Its proprietary lipid nanoparticle formulation ensures strong gene expression results while minimizing cytotoxicity—making it ideal for both routine and advanced applications, including CRISPR and RNA-based therapeutics research.
Features
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High-Efficiency Transfection of mRNA: Comparable in performance to Thermo Fisher’s Lipofectamine™ MessengerMAX™, Biofargo achieves excellent mRNA delivery with minimal toxicity—ideal for mRNA vaccines, gene editing, and protein expression studies.
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Versatile Nucleic Acid Compatibility: Enables efficient transfection of plasmid DNA, mRNA, siRNA, and other nucleic acid formats using a unified protocol.
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Superior Cell Viability: Reduces cellular stress and preserves morphology, ensuring reliable downstream analysis.
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Compatible with Serum and Antibiotics: No need to remove serum or antibiotics, simplifying workflows.
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Proven in Difficult-to-Transfect Cell Lines: Effective with primary cells, stem cells, suspension cells, and commonly used lines like HEK293, HeLa, and CHO.
Applications
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mRNA transfection and expression
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siRNA-mediated gene knockdown
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Gene editing (CRISPR/Cas9)
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Protein production
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Functional genomics and cell engineering
siRNA Transfection Assay
1. Primary rat cardiomyocytes: siRNA knockdown efficiency of 82%.
2. BMDM cells: siRNA knockdown efficiency of 60% with no detectable cytotoxicity.
3. RAW264.7 cells: siRNA knockdown efficiency of 70%.
4. U87MG cells: siRNA knockdown efficiency of 85%, accompanied by significant inhibition of cell proliferation.
Reference for Cell Transfection Efficiency
| Cell Name | Knockdown Efficiency |
| HeLa | 85% |
| U-2 OS | 85% |
| HEK293 | 85% |
| HCT-116 | 85% |
| BHK-21 | 85% |
| COS-7 | 85% |
| HT-1080 | 85% |
| NCI-H441 | 85% |
| U-87 MG | 85% |
| Glial | 80% |
| Primary Rat Cortical Cells | 80% |
| Primary Rat Hippocampal | 80% |
| HepG2 | 80% |
| A549 | 80% |
| K-562 | 80% |
| MCF7 | 80% |
| Jurkat | 80% |
| THP-1 | 80% |
| L-929 | 80% |
| 3T3 | 80% |
| Huh7 | 80% |
| NK-92 | 80% |
| Raji | 80% |
| Rat Cardiomyocytes | 80% |
| Saos-2 | 80% |
| U-937 | 80% |
| SW480 | 80% |
| L6 | 80% |
| PC-3 | 80% |
| 4T1 | 80% |
| Raw264.7 | 70% |
| HUVEC | 70% |
| Human T Cells | 70% |
| SH-SY5Y | 70% |
| Mouse Embryonic StemCells | 60% |
| PBMC | 60% |
| BMDM | 60% |
| Mesenchymal Stem Cell (hMSC) | 60% |
FAQ
Q: Can I deliver single-stranded oligonucleotides with Transfection Reagent?
A: If delivering a single-stranded oligonucleotide alone, it's recommended to use Lipofectamine RNAiMAX Transfection Reagent. However, Transfection Reagent can be used for genome editing when co-delivering Cas9 mRNA, sgRNA, and a donor template (ssDNA or dsDNA).
Q: How do I use Transfection Reagent to deliver both Cas9 mRNA and IVT gRNA together?
A: Transfection Reagent supports co-delivery of Cas9 mRNA with single or multiple gRNAs, providing high transfection efficiency. It is compatible with cleavage detection or selection kits for downstream mutation analysis.
Q: Which cell types can be used with Transfection Reagent?
A: Transfection Reagent shows low cytotoxicity and high efficiency in various cell types, including primary and stem cells, as well as those that are typically difficult to transfect.
Q: When should I use Transfection Reagent over other reagents?
A: Use Transfection Reagent for CRISPR applications requiring the co-delivery of mRNA and gRNA, along with short donor DNA templates. Its flexibility makes it ideal for HDR-based genome editing.
Q: What is Transfection Reagent?
A: Transfection Reagent is an animal-origin-free reagent formulated for delivery of mRNA, small RNA (e.g., gRNA, siRNA, miRNA), and short donor DNA (0.5–1 kb), making it highly suitable for CRISPR genome editing workflows.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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