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$19.00
Sku: BB0083-100mg
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by July 10, 2026
Description
In blue-white screening and lacZ-based detection workflows, inconsistent colony color development can make it difficult to distinguish recombinant colonies from background growth. Weak blue coloration, unstable substrate quality, or poor storage conditions may lead to ambiguous screening results, repeated plates, and wasted cloning time.
X-Gal Ultra Pure Grade is a high-purity chromogenic substrate for β-galactosidase, commonly used in blue-white colony screening, lacZ reporter systems, and molecular cloning workflows. In many cloning workflows, X-Gal is used together with Biofargo IPTG to support lac operon induction and visual colony differentiation. When cleaved by β-galactosidase, X-Gal produces an insoluble blue product, allowing researchers to identify lacZ-positive colonies or β-galactosidase activity in suitable experimental systems.
Biofargo X-Gal is supplied as an Ultra Pure grade powder with ≥99.0% HPLC purity, helping support reliable colony color differentiation and reproducible molecular biology experiments. It is suitable for laboratories that require a dependable X-Gal powder for recombinant colony screening, bacterial transformation workflows, and β-galactosidase activity detection.
Biofargo X-Gal Ultra Pure Grade is designed for research laboratories performing blue-white screening, lacZ-based detection, and molecular cloning workflows that require high-purity X-Gal substrate.
Specifications
| Product Name | X-Gal Ultra Pure Grade |
| Chemical Name | 5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside |
| Product Code | BB0083 |
| CAS Number | 7240-90-6 |
| Formula | C14H15BrClNO6 |
| Molecular Weight | 408.6 g/mol |
| Grade | Ultra Pure |
| Purity | ≥99.0% area by HPLC |
| Appearance | White to off-white crystalline powder |
| Water Content | ≤0.5% |
| Sterile | No |
| Dangerous Goods | No |
| Storage | -15 to -20°C. Keep tightly closed, dry, and protected from heat, light, and moisture. |
| Shipping Conditions | ICE |
| Refrigeration Requirements | Freezer |
| Available Sizes | 100 mg, 1 g, 5 g, 100 g |
| Number of Containers | 1 |
| UNSPSC Code | 12161500 |
| UNSPSC Category | Indicators |
Features
- Ultra Pure grade X-Gal substrate for blue-white screening and β-galactosidase detection workflows.
- High purity specification of ≥99.0% area by HPLC supports reproducible color development in molecular biology applications.
- Chromogenic β-galactosidase substrate that produces a blue insoluble product after enzymatic cleavage in suitable lacZ-positive systems.
- Useful for identifying recombinant and non-recombinant colonies in cloning workflows that use lacZ α-complementation.
- Available in multiple package sizes, from 100 mg for small-scale testing to 100 g for routine laboratory use.
- Supplied as a white to off-white crystalline powder with low water content specification for reliable storage and handling.
- Not classified as dangerous goods for transport, supporting convenient laboratory procurement and shipping.
Applications
- Blue-white colony screening in molecular cloning workflows
- Visual identification of lacZ-positive bacterial colonies
- β-galactosidase activity detection in research assays
- Screening of recombinant and non-recombinant colonies after bacterial transformation
- Colony screening workflows using X-Gal together with IPTG under suitable experimental conditions
- lacZ reporter gene detection in molecular biology research
- Cloning experiments involving lacZ α-complementation systems
- E. coli colony screening in plasmid-based cloning workflows
- Colorimetric detection of β-galactosidase expression in research applications
- Preparation of research media or plates requiring X-Gal substrate according to established laboratory protocols
Storage and Handling
Store X-Gal powder at -15 to -20°C in a tightly closed container. Keep the product dry and protected from heat, light, and moisture to help maintain substrate quality.
Allow the container to equilibrate before opening when moving from freezer storage to room-temperature handling conditions. This helps reduce moisture condensation inside the container.
Prepare and use X-Gal solutions according to the validated protocol used in your laboratory. Avoid unnecessary light exposure and repeated freeze-thaw handling of prepared aliquots when applicable.
Documents
FAQ
What is X-Gal used for in molecular biology?
X-Gal is commonly used as a chromogenic substrate for β-galactosidase. In molecular biology laboratories, it is most often used for blue-white colony screening, lacZ-based detection, and visual identification of β-galactosidase activity in suitable research systems.
How does X-Gal work in blue-white screening?
In blue-white screening, colonies with active β-galactosidase can cleave X-Gal and produce a blue-colored product. Colonies without functional β-galactosidase activity typically remain white under appropriate screening conditions, helping researchers identify candidate recombinant colonies.
Is Biofargo X-Gal suitable for colony screening with IPTG?
Yes. X-Gal is commonly used together with IPTG in blue-white colony screening workflows when the bacterial strain, plasmid system, media, and incubation conditions support lacZ-based color development.
What is the CAS number of X-Gal?
The CAS number of X-Gal, also known as 5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside, is 7240-90-6.
What purity is Biofargo X-Gal Ultra Pure Grade?
Biofargo X-Gal Ultra Pure Grade has a purity specification of ≥99.0% area by HPLC, making it suitable for research applications where consistent substrate quality is important.
How should X-Gal powder be stored?
X-Gal powder should be stored at -15 to -20°C in a tightly closed container. It should be kept dry and protected from heat, light, and moisture to help preserve product quality.
Why are some colonies pale blue or difficult to interpret when using X-Gal?
Weak or unclear colony color can be affected by substrate condition, storage history, strain background, plasmid system, incubation time, media formulation, and induction conditions. Using high-purity X-Gal and protecting the substrate from light and moisture can help reduce reagent-related variability.
Is X-Gal sterile?
Biofargo X-Gal Ultra Pure Grade is listed as non-sterile. Use appropriate aseptic handling or validated preparation methods according to your laboratory protocol when preparing solutions or media.
Is X-Gal considered dangerous goods for shipping?
Biofargo X-Gal is listed as not dangerous goods. It ships on ICE and should be transferred to freezer storage after receipt.
Which X-Gal package size should I choose?
For occasional cloning or method validation, 100 mg may be suitable. For routine blue-white screening or shared laboratory inventory, 1 g or 5 g sizes are often more practical. The 100 g size is available for high-use laboratories or larger-scale research workflows.
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Disclaimer
For laboratory research use only. Not for human or animal diagnostic or therapeutic use.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
