Viral Nervous Necrosis Virus(VNNV) Probe qRT-PCR Kit

Sensitive and Rapid Detection Kit for Viral Nervous Necrosis Virus in Aquaculture – 50 Reactions

$641.00

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Sku: 15-53200
Categories: Cell & Molecular Biology , Custom Solutions , Pathogen Detection Kits , qPCR Kit
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Description

Viral Nervous Necrosis Virus(VNNV) Probe qRT-PCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.

Key Features

One-tube qPCR format minimizes carry-over contamination risk

  • High sensitivity: analytical LOD as low as 100 copies/reaction

  • Excellent specificity: no cross-reactivity with DNA from other pathogens

  • Includes amplification positive control for result verification

  • Compatible with various DNA purification methods

  • Suitable for both qualitative and quantitative applications

  • For quantitative use: the linear range covers a 100,000-fold concentration difference.

  • Sufficient for 50 reactions (20 μL each)

  • For Research Use Only (RUO)

Kit Components

Component Name Product Number. Size Vial Lid Color
Probe qRT-PCR Buffer 990504a 500 μL 0.5 μL Vial Blue
Probe qRT-PCR Enzyme 990504bv2 50 μL 0.5 mL Vial Red
Template Dilution Buffer 180701 1 mL 1.5 mL Vial Green
Primer-Probe Powder yp15-53200 50 T 1.5 mL Brown Vial Brown
Amplification Positive Control
(1E7 copies/μL)		 pc53200 0.5 mL 0.5 mL Vial Yellow
Manual sc15-53200 1 Set NA NA

 

Application Areas

  • Aquaculture disease surveillance and diagnostics: sensitive detection and quantification of VNNV RNA in fish tissues (e.g., brain, retina, spinal cord), larvae/juveniles and broodstock for routine health screening and to prevent vertical and horizontal transmission in hatcheries and farms.
  • Research and vaccine/antiviral studies: accurate viral load monitoring in experimental infection models, challenge trials and antiviral or vaccine efficacy studies to evaluate pathogenesis and treatment outcomes.
  • Environmental monitoring and product safety: screening of rearing water, live feed, sediment and processed seafood for VNNV RNA to support biosecurity, environmental risk assessment and seafood safety compliance.

Storage & Shipping

  • Store at - 20 °C

  • The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.

FAQ

Q: Are Biofargo PCR and qPCR Kits compatible with my thermal cycler or real-time PCR instrument?

A: Yes. Biofargo PCR Kits and probe-based qPCR Kits are designed for broad compatibility with mainstream instruments.

PCR Kits can be used with standard thermal cyclers from leading brands.

Probe-based qPCR Kits are fully compatible with major real-time PCR platforms, including ABI, Bio-Rad, Roche, QuantStudio, Qiagen Rotor-Gene Q (5-plex), and Genesig Q16.

Our technical team is available to assist with instrument setup and run optimization to ensure the best results.

Documents

Viral Nervous Necrosis Virus(VNNV) Probe qRT-PCR Kit User Manual

MSDS

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

Terms and Conditions

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