U+ HS Probe Multiplex qPCR Mix (UDG)——StarLighter

Description

Multiplex probe-based qPCR laboratories often face two challenges at the same time. First, the detection of low-copy or single-copy targets demands very high sensitivity from the reagent. Second, in repeated multiplex runs, residual amplification products can contaminate new reactions and cause false positives — and in high sensitivity multiplex detection, contamination is exactly what can mask the real signal of the target.

StarLighter U+ HS Probe Multiplex qPCR Mix is a 2× premix that contains all components except the template, primers and probes. It combines a high-performance hot-start Taq Pro DNA polymerase with a buffer reaction system optimized for multiplex reactions, effectively inhibiting non-specific amplification and greatly increasing qPCR amplification efficiency and the sensitivity of multiplex detection. The UDG version (catalog No. FS-Q8003) introduces a dUTP/UDG anti-contamination system that works at room temperature, eliminating the impact of amplification-product contamination on qPCR results and ensuring accuracy. This high sensitivity multiplex probe qPCR master mix reaches a detection limit as low as a single copy.

To set up a reaction, simply add primers, template, probes, ROX (according to the instrument used) and water — the ROX reference dye is supplied with the kit. The kit provides good amplification curves across a wide concentration range, is suitable for quantification, qualitative detection and SNP detection of multiple target genes, offers good repeatability and high reliability, and is compatible with various types of qPCR probes.

Specification

Format: 2× premix containing all components except template, primers and probes. The ROX reference dye is supplied with the kit and added according to the instrument used. The UDG version (FS-Q8003) contains a dUTP/UDG anti-contamination system that is active at room temperature.

Features

• Detection limit as low as a single copy — high sensitivity multiplex probe qPCR master mix for rare and low-abundance targets.
• Room-temperature dUTP/UDG anti-contamination system — eliminates the impact of amplification-product contamination on qPCR and ensures accurate results; UDG removal efficiency is higher than 99.9% (a difference of 10 Ct values corresponds to 99.9% removal efficiency), verified by quantifying the same dUTP-containing amplicon template with the UDG version (FS-Q8003) and a non-UDG version of the mix.
• Hot-start Taq Pro DNA polymerase — effectively inhibits non-specific amplification and greatly increases qPCR amplification efficiency and multiplex detection sensitivity.
• Multiplex performance with an internal control — in quadruplex detection of IPC (internal control, high template) together with hPRT1 (low GC), CSTB (high GC) and F8 (low GC) at 0.1 ng input each (about 30 copies), this master mix shows lower Ct values and higher fluorescence signals than a competitor product.
• Low-concentration pathogen target detection — in quadruplex detection of IPC and African swine fever virus (ASFV) at about 20 copies, this master mix shows lower Ct values and higher fluorescence signals than a competitor product.
• Wide linear range and higher detection sensitivity — in quadruplex detection of hPRT1, ApoE, actin and F8 with template inputs from 200 ng down to 0.0061 ng (six points), this master mix shows higher detection sensitivity and fluorescence signal intensity, with lower Ct values, than another competitor multiplex probe qPCR mix.
• Good batch-to-batch consistency — three batches show consistent fluorescence intensity and Ct values in quadruplex quantitative amplification (10 ng, 1 ng and 0.1 ng inputs).
• Broad primer/probe compatibility — primers and probes with different GC% and Tm values amplify well, compatible with various types of qPCR probes.
• Good amplification curves across a wide concentration range — supports accurate quantification of multiple target genes with good repeatability and high reliability.
• Simple 2× premix format — just add primers, template, probes, ROX (according to the instrument) and water.

Application

StarLighter U+ HS Probe Multiplex qPCR Mix (UDG) is suitable for:

• Gene expression analysis — quantification of multiple target genes in a single multiplex reaction.
• Low copy gene detection — sensitive detection of rare transcripts and low-abundance targets.
• SNP and allele typing — qualitative and genotyping assays on multiple targets.

FAQ