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For laboratory research use only.
$16.00
Sku: TG217(G211)-100g
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by July 02, 2026
Description
In microbiological culture preparation, inconsistent nitrogen sources often lead to unstable bacterial growth, poor batch-to-batch reproducibility, and unpredictable fermentation performance. Researchers working with LB medium, diagnostic culture systems, or industrial fermentation frequently encounter variability caused by low-quality enzymatic digests or poorly controlled peptone raw materials.
Tryptone Powder (TG217) is a bacteriological-grade enzymatic digest of casein designed to provide a consistent and biologically available source of peptides and amino nitrogen for microbial growth. It is widely used in microbiology, fermentation, recombinant protein expression, and diagnostic media formulation where reproducibility and nutrient consistency are critical.
This product delivers a controlled profile of amino acids, peptides, and nitrogen compounds that support robust microbial proliferation in both routine laboratory workflows and scalable bioprocess applications. It is commonly used as a primary nitrogen source in LB broth and other complex culture media formulations requiring high growth performance and low variability between batches.
Manufactured under controlled processing conditions and tested for microbiological quality, TG217 Tryptone Powder is optimized for laboratory environments requiring reliable performance, low contamination risk, and consistent nutrient composition across production-scale and research-scale applications.
Specification
| Chemical & Physical Properties | |
|---|---|
| Appearance | Fine, homogenous, light to medium tan yellow powder |
| Amino Nitrogen (AN) | ≥ 3.9% |
| Total Nitrogen (TN) | ≥ 10% |
| Loss on Drying | ≤ 6.0% |
| Ash Content | ≤ 15% |
| pH (2% solution) | 6.5 – 7.5 |
| Microbiological Quality | |
| Standard Plate Count | ≤ 5000 CFU/g |
| Molds & Yeast | ≤ 100 CFU/g |
| Coliforms | Negative |
| Salmonella | Negative |
| Functional Performance | |
| Peptone Agar (USP) | Satisfactory growth performance |
| Storage | |
| Condition | Room Temperature (dry and sealed) |
Features
-
Consistent enzymatic casein hydrolysate for reproducible microbial culture performance
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High total nitrogen content (≥10%) supporting robust bacterial growth
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Balanced amino nitrogen profile (≥3.9%) for stable metabolic activity
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Low bioburden specification suitable for contamination-sensitive workflows
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Validated performance in USP peptone agar systems
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Optimized solubility for rapid media preparation and batch consistency
-
Suitable for both research-scale and industrial fermentation systems
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Manufactured under controlled thermal processing conditions for stability
Applications
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LB broth and complex microbial culture media preparation
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Escherichia coli cultivation and recombinant protein expression systems
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Industrial fermentation and microbial bioprocess development
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Diagnostic microbiology media formulation
-
Enzyme production and metabolite biosynthesis workflows
-
Pharmaceutical and veterinary microbiology research
-
General bacterial growth media optimization studies
Documents
Regulatory Notice: For laboratory research use only. Not intended for diagnostic or therapeutic use.
Frequently Asked Questions
What is tryptone powder used for in microbiology?
Tryptone powder is used as a nitrogen-rich enzymatic digest of casein in microbial culture media. It supports bacterial growth by providing peptides and amino acids required for metabolism, commonly in LB broth and fermentation media.
Is tryptone the same as peptone?
Tryptone is a specific type of peptone produced by enzymatic digestion of casein, while peptone is a broader category that may come from different protein sources such as meat, soy, or casein.
Can tryptone be used in LB medium preparation?
Yes. Tryptone is one of the key components in LB (Luria-Bertani) medium, providing essential nitrogen and peptides that support E. coli and other bacterial growth.
What is the difference between tryptone and casein hydrolysate?
Tryptone is a refined enzymatic hydrolysate of casein with controlled peptide composition, while general casein hydrolysates may have less consistent peptide distribution and performance.
Why is nitrogen content important in tryptone?
Nitrogen content determines the availability of amino acids and peptides for microbial metabolism. Higher and stable nitrogen levels improve reproducibility in fermentation and culture media performance.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
