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PEG T-Octylphenyl Ether (N = 9-10)
$24.00
Sku: TB0198-500mL
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by July 09, 2026
Description
In molecular biology and cell-based research workflows, inconsistent cell disruption and inefficient membrane solubilization often lead to low protein yield, poor reproducibility, and reduced sensitivity in downstream assays such as Western blotting, immunofluorescence (IF), and protein interaction analysis. Triton X-100 (TB0198), a nonionic detergent (non-ionic surfactant), is designed to provide controlled membrane permeabilization and efficient cell lysis while maintaining native protein structure and functional integrity.
As a PEG t-octylphenyl ether-based laboratory detergent (CAS 9002-93-1), Triton X-100 is widely used in biochemical and molecular biology applications requiring mild, non-denaturing solubilization of lipid membranes and membrane-associated proteins.
Specification
| Product Name | Triton X-100 |
| Product Code | TB0198 |
| CAS Number | 9002-93-1 |
| Molecular Weight | 647 g/mol |
| Grade | Biotech Grade |
| Appearance | Light yellow liquid |
| Storage | 18–25°C |
| Shipping Condition | Room Temperature (RT) |
Features
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Mild non-ionic detergent performance:
Functions as a non-ionic surfactant that disrupts lipid-lipid and lipid-protein interactions without strongly denaturing proteins.
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Efficient cell lysis reagent:
Enables reproducible cell lysis and intracellular protein extraction across mammalian, bacterial, and cultured cell systems.
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Controlled membrane permeabilization:
Provides optimized membrane permeabilization for immunofluorescence (IF), ICC, and intracellular antibody staining.
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High compatibility with protein assays:
Compatible with Western blot (WB), ELISA, and enzymatic activity assays without significant interference.
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Membrane protein solubilization:
Effectively solubilizes membrane proteins while maintaining functional conformation for downstream analysis.
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Lipid raft research compatible:
Suitable for membrane fractionation and lipid raft isolation in signal transduction studies.
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Stable laboratory detergent system:
Exhibits consistent performance in aqueous buffer systems for biochemical and molecular biology workflows.
Applications
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Cell lysis and protein extraction:
Used as a standard cell lysis reagent for extracting cytoplasmic and membrane-associated proteins.
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Membrane permeabilization:
Widely used in immunofluorescence (IF) and immunocytochemistry (ICC) for intracellular antigen detection.
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Western blot sample preparation:
Improves protein solubilization and reduces background in protein detection workflows.
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Protein purification:
Supports extraction of membrane proteins while preserving functional structure for biochemical studies.
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Lipid raft and membrane biology:
Applied in membrane fractionation and lipid raft isolation for signal transduction research.
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Molecular biology assays:
Used in ELISA preparation, enzyme assays, and biochemical protein interaction studies.
Documents
Frequently Asked Questions
What is Triton X-100 used for in cell biology?
Triton X-100 is commonly used as a nonionic detergent for cell lysis, membrane permeabilization, and protein extraction in molecular biology and biochemical research workflows.
Is Triton X-100 a harsh or mild detergent?
It is a mild, non-denaturing detergent that disrupts lipid membranes while preserving most protein structures, making it suitable for functional protein studies.
What experiments commonly use Triton X-100?
It is widely used in Western blotting, immunofluorescence (IF), immunocytochemistry (ICC), protein purification, and membrane protein solubilization studies.
What is the difference between Triton X-100 and SDS?
Triton X-100 is a nonionic mild detergent used for membrane solubilization, while SDS is an ionic strong detergent that denatures proteins for electrophoresis applications.
Can Triton X-100 be used in immunofluorescence protocols?
Yes. It is widely used to permeabilize cell membranes, allowing antibodies to access intracellular targets during immunofluorescence and immunocytochemistry.
For laboratory research or industrial use only.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
