Tri-Color Prestained Protein Marker (10–180 kDa)

Description

Tri-Color Prestained Protein Marker (10–180 kDa) is a ready-to-use protein molecular weight marker designed for accurate protein size estimation during SDS-PAGE and Western blot workflows. In protein electrophoresis experiments, unclear band positioning, inconsistent molecular weight estimation, and difficulty monitoring membrane transfer can affect downstream protein analysis. A reliable prestained protein ladder provides researchers with a visible reference standard to evaluate protein migration and transfer performance.

This tri-color prestained protein marker contains 10 colored protein bands covering a broad molecular weight range from 10 kDa to 180 kDa. The marker produces clear visual bands directly during gel electrophoresis and after transfer onto PVDF or nitrocellulose (NC) membranes, making it suitable as a protein molecular weight marker for Western blotting and SDS-PAGE applications.

The three-color design includes a green 10 kDa reference band, a red 70 kDa reference band, and blue bands for the remaining molecular weight positions. Compared with traditional single-color protein ladders, this multicolor protein marker enables faster identification of protein regions and improves experimental convenience in routine molecular biology workflows.

Tri-Color Prestained Protein Marker is supplied as a ready-to-use liquid formulation that requires no heating, dilution, or addition of reducing agents before loading. It is suitable for laboratories performing protein electrophoresis, recombinant protein analysis, and Western blot detection.

Specifications

Features

• Tri-color prestained protein ladder: Provides clearly distinguishable colored bands for rapid protein migration monitoring and molecular weight estimation.

• Broad molecular weight coverage: Contains 10 protein bands ranging from 10 kDa to 180 kDa, suitable for a wide range of protein analysis applications.

• Ready-to-use formulation: Direct loading without heating, dilution, or additional reducing agents simplifies SDS-PAGE sample preparation.

• Compatible with SDS-PAGE and Western blot: Enables visualization during electrophoresis and after transfer to PVDF or NC membranes.

• Stable long-term performance: Maintains reliable band quality during long-term storage at -20 °C.

• Easy protein size identification: Dedicated colored reference bands help researchers quickly locate target protein regions.

Applications

Tri-Color Prestained Protein Marker is widely used as a protein electrophoresis standard for molecular biology, biochemical research, and protein analysis workflows.

• Western Blot Protein Detection: Used as a prestained Western blot protein marker to monitor protein transfer efficiency and estimate target protein molecular weight after membrane transfer.

• SDS-PAGE Protein Electrophoresis: Provides visible molecular weight references during gel electrophoresis for protein separation and analysis.

• Protein Molecular Weight Estimation: Helps determine approximate sizes of unknown proteins by comparing sample bands with known marker positions.

• Recombinant Protein Analysis: Supports verification of recombinant protein expression and purification results.

• Cell Lysate Protein Analysis: Suitable for evaluating protein profiles from cultured cells and biological samples.

• Protein Transfer Monitoring: Allows researchers to visually confirm protein migration from gel to PVDF or NC membranes.

• Protein Electrophoresis Teaching and Training: Suitable for educational laboratories requiring clear visualization of protein standards.

Protocol Overview

Tri-Color Prestained Protein Marker is supplied as an immediate-use protein ladder for SDS-PAGE and Western blot workflows.

• Gently mix the marker solution before use to ensure uniform distribution of protein bands.
• Load 3–5 μL of the marker directly into the SDS-PAGE gel.
• Perform electrophoresis according to standard protein separation procedures.
• Visualize colored marker bands during electrophoresis or after transfer onto PVDF/NC membranes.

No heating, dilution, or addition of reducing agents is required before loading.

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