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Description

Efficient viral vector production requires a reliable HEK293 suspension culture system that supports high-density cell growth and effective transient transfection. Maintaining suitable cell culture conditions is important for achieving consistent performance during AAV and lentiviral vector production workflows.

CelliMax® TransV-293 Kit03 is a GMP-grade chemically defined HEK293 suspension medium system designed for high-density suspension culture of different HEK293 subtypes. The complete kit contains basal medium, feed supplements, and transfection reagent to support HEK293 culture and transient transfection applications.

This chemically defined medium system is free of animal-derived components, hydrolysates, and growth factors. CelliMax® TransV-293 Kit03 provides a specialized solution for researchers and bioprocess developers working with HEK293 suspension culture, AAV production, and lentiviral vector production applications.

Product Information

Product Name Type SKU Specification Grade
CelliMax® TransV-B03 Basal Medium TSVHEK02 1 L GMP Grade
CelliMax® TransV-FM03A Primary Feed Medium
CelliMax® TransV-FM03B Supplementary Medium
CelliMax® TransV-Reagent Transfection Reagent

 

Features

  • GMP-grade chemically defined (CD) medium system.

  • Free of animal-derived components, hydrolysates, and growth factors.

  • Suitable for high-density suspension culture of different HEK293 subtypes.

  • Supports transient transfection with reported transfection efficiency greater than 90%.

  • Designed for AAV and lentiviral vector production applications.

  • Requires low transfection reagent usage to reduce cytotoxic effects.

Product Performance

CelliMax® TransV-293 Kit03 performance data demonstrates support for HEK293 suspension culture with maintained cell viability during culture.

  • AAV viral titer up to 10¹¹ vg/mL.

  • Lentiviral vector titer up to 10⁷ TU/mL.

  • AAV samples showed titers from 2.36 × 10¹¹ vg/mL to 3.01 × 10¹¹ vg/mL with 95% purity in reported testing samples.

Applications

AAV Production

CelliMax® TransV-293 Kit03 is suitable for HEK293 suspension culture workflows involving AAV production. The system provides a chemically defined culture environment for applications requiring AAV viral vector generation.

Lentiviral Vector Production

The kit supports lentiviral vector production applications using HEK293 suspension culture and transient transfection workflows.

HEK293 Suspension Culture

Designed for high-density suspension culture of different HEK293 subtypes, CelliMax® TransV-293 Kit03 provides a complete culture system containing basal medium, feed supplements, and transfection reagent.

Transient Transfection Applications

The included CelliMax® TransV-Reagent supports transient transfection workflows with reported transfection efficiency greater than 90%.

Specifications

Medium Type Chemically Defined (CD)
Culture Format HEK293 Suspension Culture
Grade GMP Grade
Specification 1 L
Application AAV Production, Lentiviral Vector Production, HEK293 Transient Transfection

 

FAQ

What type of cells is CelliMax® TransV-293 Kit03 designed for?

CelliMax® TransV-293 Kit03 is designed for high-density suspension culture of different HEK293 subtypes.

Is CelliMax® TransV-293 Kit03 a chemically defined medium?

Yes. The system is a GMP-grade chemically defined (CD) medium system free of animal-derived components, hydrolysates, and growth factors.

What applications are supported by CelliMax® TransV-293 Kit03?

The product supports HEK293 suspension culture, transient transfection, AAV production, and lentiviral vector production applications.

What components are included in the kit?

The kit includes basal medium, primary feed medium, supplementary medium, and transfection reagent.

What is the reported AAV viral titer performance?

According to product performance data, AAV viral titer can reach up to 10¹¹ vg/mL.

 

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

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