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Description
This kit utilizes a unique lysis buffer combined with a specially optimized, efficient humic acid precipitation agent to extract genomic DNA from various microorganisms, including Gram-negative bacteria, Gram-positive bacteria, and fungi, present in diverse environmental samples such as soil, feces, fermentation products, water, pond sludge, marine sediment, and gut contents.
The grinding beads and homogenizer can effectively crush the microorganisms in the environmental samples to ensure the high efficiency and integrity of the extracted genomic DNA.
Genomic DNA extracted by this kit has few impurities and good integrity, and can be directly used in downstream experiments of molecular biology such as PCR, database building, and sequencing.
Kit Contents
| Contents | GDP713 (96 preps) | |
| DP713 | Buffer SNA | 80 mL |
| Buffer SNE | 80 mL | |
| HA Removal Buffer CN | 30 mL | |
| Buffer LB4A | 40 mL | |
| Buffer W2 | 160 mL | |
| Buffer RW | 40 mL | |
| Buffer TB | 15 mL | |
| Buffer TE | 15 mL | |
| RNase A (10 mg/mL) | 1 mL | |
| MagAttract Suspension BE (100 mg/mL) | 2 × 1 mL | |
| OSE-TH-B08 | Envir-DNA Grinding Beads Tube | 2 × 48 tubes / pack |
Features
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Quick and simple: Quickly remove humic acid in 1 minute.
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High purity: The extracted DNA is of high purity and can be used directly in downstream experiments.
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Wide application: Suitable for microbial genomic DNA extraction from many types of environmental samples.
Applications
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Soil samples (common soil, high humic acid soil, lean soil, difficult soils)
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Fecal samples (mouse, livestock, poultry, gut contents)
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Water samples and fermentation broths
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Pond sludge, lake sediment, deep-sea sediment
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Air and water filter membranes
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Plant, fungal, and tissue-associated environmental samples
Storage
The kit can be stored for 15 months at room temperature (15–30 °C) under dry conditions.
If precipitation occurs, preheat the solution in a 37 °C water bath for 10 minutes before use to dissolve the precipitate without affecting performance.
Notes
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Newly collected samples usually yield higher DNA amounts. Refer to optimal preservation conditions for different sample types before sampling.
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Excess DNA may inhibit downstream PCR reactions. If inhibition occurs, dilute the DNA template before use.
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HA Removal Buffer CN should be pre-chilled at 2–8 °C before use to achieve optimal humic acid removal efficiency.
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Two elution buffers are provided:
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Buffer TB: Does not contain EDTA
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Buffer TE: Contains EDTA (recommended for long-term storage, but EDTA may inhibit PCR and library construction)
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Due to the complexity of environmental samples, extracted nucleic acid concentrations may be low and degradation may occur during long-term storage. It is recommended to perform downstream experiments as soon as possible after extraction.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
