TIANamp Genomic DNA Kit-Tiangen

Description

The TIANamp Genomic DNA Kit provides a fast and easy silica-based format for DNA purification. The kit simplifies the purification of DNA from a wide range of sample types, including animal blood (≤ 1 ml), cultured cells, and tissues commonly encountered in life science, veterinary, and genotyping applications. Most samples could be directly lysed with the provided buffer system, eliminating the need for mechanical disruption and also reducing hands-on time. The kit purifies high-quality DNA without proteins and inhibitors. The purified DNA is suitable for PCR, restriction digestion and Southern blot.

Required Reagents

Red Cell Lysis Buffer (for blood samples more than 200 μl, Cat# 4992810), ethanol, RNase A (optional)

Features

• Silica-membrane technology provides fast way to purify ready-to-use high quality gDNA.

• High DNA yield, excellent repeatability.

• This kit significantly removes contaminants and inhibitors, facilitating downstream applications.

Important Notes

• Please use fresh samples to ensure the integrity of the purified gDNA.

• If the sample starting amount exceeds the recommended range of the kit, it is recommended to increase the amount of Buffer GA and Buffer GB, and split the column loading in separate times, or perform the experiment using two spin columns.

• For long-term storage of the gDNA, it is recommended to store in buffer TE. Please ensure that the OD260/OD280 value is in the range of 1.7-1.9, and the concentration of gDNA is 100-300 ng/μl.

DNA Extraction Yield From Various Plant Tissues

Storage

Room temperature (15-25℃)

Publications

Dual-responsive nanocarriers for efficient cytosolic protein delivery and CRISPR-Cas9 gene therapy of inflammatory skin disorders.

Publication: Science Advances