Three-color Prestained Protein Marker (10–250 kDa)

Description

Three-color Prestained Protein Marker (10–250 kDa) is a ready-to-use protein molecular weight marker designed for accurate protein size estimation during SDS-PAGE and Western blot workflows. During protein electrophoresis experiments, unclear band positioning, inconsistent molecular weight estimation, and difficulty monitoring membrane transfer efficiency can affect downstream protein analysis. A reliable prestained protein ladder provides a visible reference standard to help researchers evaluate protein migration, gel separation performance, and transfer results.

This three-color protein marker contains 10 prestained protein bands covering a broad molecular weight range from 10 kDa to 250 kDa. The color-coded design includes a green 10 kDa reference band, an orange 70 kDa reference band, and blue reference bands at other molecular weights, enabling rapid visualization of protein separation during electrophoresis. The marker can be directly loaded onto polyacrylamide gels without additional staining procedures and is suitable as a protein molecular weight marker for routine laboratory workflows.

Compared with traditional unstained protein standards, this prestained protein ladder allows researchers to monitor protein migration in real time during SDS-PAGE and provides a convenient reference for Western blot membrane transfer evaluation. Three-color Prestained Protein Marker (10–250 kDa) is suitable for protein expression analysis, recombinant protein characterization, cell lysate analysis, and general protein electrophoresis applications.

This protein marker is supplied in a ready-to-use format and requires no dilution or heating before loading, making it an efficient solution for laboratories performing frequent SDS-PAGE and Western blot experiments.

Specifications

Features

• Three-color visual identification: Color-coded protein bands provide rapid visualization of molecular weight positions during SDS-PAGE and Western blot workflows.

• Broad molecular weight coverage: Covers a range of 10–250 kDa, supporting analysis of a wide variety of proteins from small proteins to high molecular weight targets.

• Ready-to-use protein ladder: Supplied in a convenient format that can be directly loaded after thawing and mixing, reducing preparation steps before electrophoresis.

• Reliable reference for Western blot: Helps researchers evaluate protein transfer efficiency and identify membrane transfer performance during blotting workflows.

• Stable performance: Maintains product stability under recommended storage conditions for consistent use in routine protein analysis.

• Compatible with common protein electrophoresis workflows: Suitable for SDS-PAGE gels and standard Western blot procedures used in molecular biology laboratories.

Applications

Three-color Prestained Protein Marker (10–250 kDa) is widely used as a protein electrophoresis standard in molecular biology, biochemistry, and life science research laboratories. The visible reference bands provide convenient support for protein separation analysis and Western blot workflows.

• Western Blot Protein Analysis: Used as a Western blot marker to estimate target protein molecular weight and monitor protein transfer from gel to membrane.

• SDS-PAGE Protein Electrophoresis: Provides visible molecular weight references for evaluating protein migration and electrophoretic separation performance.

• Protein Molecular Weight Estimation: Enables approximate determination of target protein size by comparing sample bands with known protein standards.

• Cell Lysate Analysis: Supports protein expression analysis from whole-cell lysates prepared from cultured cells.

• Recombinant Protein Characterization: Used during recombinant protein expression and purification workflows to evaluate protein size and purity.

• Protein Transfer Monitoring: Helps researchers visually confirm transfer efficiency during Western blot membrane preparation.

• Protein Electrophoresis Quality Control: Provides a consistent molecular weight reference for routine laboratory protein analysis.

• Molecular Biology Research: Suitable for general protein detection workflows involving SDS-PAGE, immunoblotting, and biochemical studies.

Protocol Overview

Three-color Prestained Protein Marker (10–250 kDa) is supplied as a ready-to-use protein ladder for SDS-PAGE and Western blot applications. Proper handling before electrophoresis helps maintain clear band appearance and consistent migration performance.

• Allow the protein marker to equilibrate to room temperature before use.

• Mix gently by vortexing or pipetting to ensure uniform distribution of prestained protein bands.

• Load approximately 5 μL of the protein molecular weight marker per lane for routine SDS-PAGE analysis.

• Perform electrophoresis using an appropriate polyacrylamide gel concentration according to the expected molecular weight range of target proteins.

• For low molecular weight proteins below 25 kDa, higher percentage separation gels (≥15%) are recommended to improve band resolution.

• For Western blot workflows, optimize transfer conditions according to protein size. High molecular weight proteins may require extended transfer time to achieve efficient membrane transfer.

Because prestained protein markers contain dyes covalently associated with proteins, their electrophoretic migration may differ slightly from unstained protein standards. Therefore, this protein ladder should be used for approximate molecular weight estimation rather than precise molecular mass determination.

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