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N[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid
Synonym(s): 2-[(2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)amino]ethanesulfonic acid, N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid, TES free acid, OmniPur® TES
Description
TES (2-[(2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)amino]ethanesulfonic acid) is a high-purity biochemical-grade buffering reagent supplied as a white crystalline powder. Its molecular formula is C₆H₁₅NO₆S, with a molecular weight of 229.25 and CAS number 7365‑44‑8. TES dissolves readily in water to form a clear, colorless solution, making it ideal for preparing stable, pH-controlled buffers in biochemical, molecular biology, and cell biology experiments.
Specifications
| Item | Value |
| Chemical Name | N[Tris(Hydroxymethyl)Methyl]-2-Aminoethanesulfonic Acid |
| Appearance | White Crystalline Powder |
| CAS No. | 7365-44-8 |
| Molecular Formula | C6H15NO6S |
| Molecular Weight | 229.25 |
| Grade | Biochemical Grade |
| Purity | ≥99.0% |
| Moisture | ≤1.0% |
| Water Solubility | Colorless, Clear, Transparent |
| pH | 3.5–5.0 |
| Infrared Spectrum | Complies with Specifications |
| Ignition Residue | ≤0.1% |
| A260 (in 1M Water) | ≤0.050 |
| A280 (in 1M Water) | ≤0.040 |
| Heavy Metals | ≤5 ppm |
Features
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High Purity: ≥99.0% biochemical grade ensures minimal impurities for accurate experimental results.
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Effective pH Buffering: Provides stable buffering in the pH range of 7.2–7.8.
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Excellent Solubility: Forms clear, transparent solutions suitable for sensitive assays.
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Low Optical Absorbance: A260 and A280 values are controlled, minimizing interference in protein and nucleic acid measurements.
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Low Metal Content: Heavy metals ≤5 ppm, suitable for biochemical and molecular biology applications.
Applications
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Preparation of biochemical buffers for enzyme assays, protein chemistry, and molecular biology workflows.
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Cell culture media formulation requiring stable physiological pH.
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Molecular biology experiments where low optical absorbance and chemical purity are critical.
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General laboratory buffer systems ensuring reproducible pH control for research experiments.
Safety Disclaimer
This product is intended for laboratory research use only. Not for drug, human, or veterinary use. Review the Safety Data Sheet (SDS) provided by BioFargo for detailed handling, storage, hazard, and disposal information. Use appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection when handling. Dispose of waste and residual buffer in accordance with local, state, and federal regulations. Ensure proper ventilation if preparing large volumes or heating solutions.
Documents
MSDS
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.

