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Sku: FS-T1008-V2-S
ssDNA Assay Kit for Single-Stranded DNA Quantification——StarLighter
Description
Laboratories quantifying oligonucleotides or long single-stranded DNA may require greater sensitivity and accuracy than traditional UV A260 measurements provide. The StarLighter ssDNA Assay Kit offers an easy fluorescence-based solution for single-stranded DNA quantification across a total assay range of 1–200 ng.
Depending on the sample volume, this ssDNA quantification kit accurately detects initial ssDNA concentrations from 50 pg/µL to 200 ng/µL. It can be used to measure oligonucleotides or long ssDNA and is compatible with both a Qubit fluorometer and fluorescence microplate readers.
Common contaminants, including salts, free nucleotides, solvents, detergents, and proteins, are well tolerated by the assay. Nucleotides and short oligonucleotides of six bases or fewer do not interfere with quantification. The StarLighter ssDNA reagent also exhibits fluorescence enhancement when bound to double-stranded DNA and RNA, which should be considered when evaluating samples containing these nucleic acids.
Specification
| Product Name | StarLighter ssDNA Assay Kit |
|---|---|
| Product Type | Fluorescent ssDNA Quantification Kit |
| Target | Oligonucleotides or long single-stranded DNA |
| Initial ssDNA Concentration Range | 50 pg/µL–200 ng/µL, depending on sample volume |
| Total Assay Range | 1–200 ng |
| Sensitivity | 1 ng |
| Detection Method | Fluorescence-based quantification |
| Compatible Instruments | Qubit fluorometer or fluorescence microplate reader |
| Contaminant Tolerance | Salts, free nucleotides, solvents, detergents, and proteins are well tolerated |
| Short Oligonucleotide Interference | Nucleotides and short oligonucleotides of six bases or fewer do not interfere |
| Other Nucleic Acid Response | The reagent exhibits fluorescence enhancement when bound to dsDNA and RNA |
Ordering Information
| Description | Catalog Number | Size |
|---|---|---|
| StarLighter ssDNA Assay Kit | FS-T1008-V2-S | 100 assays |
| StarLighter ssDNA Assay Kit | FS-T1008-V2 | 1000 assays |
Features
- Fluorescence-based single-stranded DNA quantification
- Total ssDNA assay range of 1–200 ng
- Initial ssDNA concentration range from 50 pg/µL to 200 ng/µL, depending on sample volume
- Detection of oligonucleotides and long ssDNA
- Compatible with a Qubit fluorometer
- Compatible with fluorescence microplate readers
- High sensitivity with a stated sensitivity of 1 ng
- Excellent tolerance to common contaminants
- Easy and fast workflow
- Available in 100-assay and 1000-assay configurations
Application
The StarLighter ssDNA Assay Kit is designed for accurate fluorescent quantification of single-stranded DNA across a total assay range of 1–200 ng. It supports the following applications:
- Single-stranded DNA quantification
- Oligonucleotide quantification
- Long ssDNA quantification
- Fluorescence-based ssDNA measurement with a Qubit fluorometer
- Fluorescence-based ssDNA measurement with a fluorescence microplate reader
FAQ
What is the quantification range of the StarLighter ssDNA Assay Kit?
The total assay range is 1–200 ng. Depending on sample volume, the assay detects initial ssDNA concentrations from 50 pg/µL to 200 ng/µL.
What types of single-stranded DNA can the assay detect?
The assay detects oligonucleotides and long single-stranded DNA.
Can the ssDNA Assay Kit be used with a Qubit fluorometer?
Yes. The StarLighter ssDNA Assay Kit is compatible with a Qubit fluorometer.
Can the assay be used with a fluorescence microplate reader?
Yes. The assay is compatible with fluorescence microplate readers.
Do nucleotides or short oligonucleotides interfere with the assay?
Nucleotides and short oligonucleotides of six bases or fewer do not interfere with the quantification assay.
Do dsDNA and RNA affect ssDNA quantification?
The StarLighter ssDNA reagent exhibits fluorescence enhancement when bound to double-stranded DNA and RNA. The possible presence of dsDNA or RNA should therefore be considered when evaluating ssDNA samples.
Which contaminants are tolerated by the assay?
Common contaminants, including salts, free nucleotides, solvents, detergents, and proteins, are well tolerated by the assay.
Which pack sizes are available?
Catalog number FS-T1008-V2-S contains 100 assays. Catalog number FS-T1008-V2 contains 1000 assays.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
