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Specification
| Package | 100 RXNS |
| Full name | SpeedyCut SfiI |
| Applications | Fast enzymatic digestion |
| Summary | SpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps. |
| Features | All SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors. |
| Composition | 100 ul enzyme, 10X SpeedyOne buffer |
| Recognition Site | 5'…-GGCCNNNN↓NGGCC-…3' 3'…-CCGGN↑NNNNCCGG-…5' |
| Reaction Buffer | 10X SpeedyOne Buffer |
| Recommended Reaction Temperature | 50°C |
| Unit Definition | At 50°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg p615 DNA in 15 minutes. |
| Methylation Effects | Dam: Methylation does not affect; Dcm: Sequences overlap completely, cleavage blocked; CpG: Completely overlapping sequences, cleavage blocked; EcoKI: Methylation does not affect; EcoBI: Methylation does not affect. |
| Thermal Inactivation | Do not heat inactivate, use phenol chloroform extraction or column purification |
| Component | 100 ul enzyme, 10X SpeedyOne buffer |
Storage
-20°C
Documents
https://cdn.shopifycdn.net/s/files/1/0521/5312/2997/files/P310003_EN_P.pdf
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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