Zea mays ZSSIIb Gene Probe Realtime PCR Kit

Description

Zea mays ZSSIIb Gene Probe Realtime PCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.

Key Features

One-tube qPCR format minimizes carry-over contamination risk

• High sensitivity: analytical LOD as low as 100 copies/reaction

• Excellent specificity: no cross-reactivity with DNA from other pathogens

• Includes amplification positive control for result verification

• Instrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc.

• Suitable for both qualitative and quantitative applications

• For quantitative use: the linear range covers a 100,000-fold concentration difference.

• Sufficient for 50 reactions (20 μL each)

• For Research Use Only (RUO)

Kit Components

Application Areas

• Quality Control of Aquaculture Feed Formulations: Quantitative monitoring of Zea mays ZSSIIb gene to ensure precise maize meal inclusion in shrimp, abalone and finfish diets—supporting feed consistency, nutritional value and supply‐chain traceability.
• Environmental DNA (eDNA) Surveillance in Coastal Ecosystems: Sensitive detection of maize‐derived genetic residues in seawater and sediment to evaluate agricultural runoff impacts on marine biodiversity and inform sustainable fisheries management.
• Regulatory Compliance & Traceability in Seafood Processing: Verification and quantification of maize‐based ingredients in processed seafood products and by‐products, ensuring adherence to food safety regulations, labeling standards and quality assurance protocols.

Storage & Shipping

• Store at - 20 °C

• The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.
  

Documents

Zea mays ZSSIIb Gene Probe Realtime PCR Kit User Manual

MSDS