Yersinia enterocolitica Probe qPCR Kit

Description

Yersinia enterocolitica Probe qPCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.

Key Features

One-tube qPCR format minimizes carry-over contamination risk

• High sensitivity: analytical LOD as low as 100 copies/reaction

• Excellent specificity: no cross-reactivity with DNA from other pathogens

• Includes amplification positive control for result verification

• Instrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc.

• Suitable for both qualitative and quantitative applications

• For quantitative use: the linear range covers a 100,000-fold concentration difference.

• Sufficient for 50 reactions (20 μL each)

• For Research Use Only (RUO)

Kit Components

Application Areas

• Aquaculture Disease Surveillance & Health Management: Rapid, quantitative detection of Yersinia enterocolitica in farmed species (abalone, shrimp, finfish) to enable early warning, optimize biosecurity protocols and support antimicrobial stewardship in commercial aquaculture operations.
• Seafood Safety & Quality Control: High-throughput screening of shellfish, fish fillets and other marine products for pathogenic Y. enterocolitica to ensure compliance with global food safety standards (FDA, EU), mitigate contamination risks and streamline HACCP-driven workflows.
• Marine Environmental Monitoring & Ecological Research: Sensitive quantification of Y. enterocolitica in seawater, sediments and marine biota for microbial risk assessment, pathogen ecology studies and coastal ecosystem surveillance programs.

Storage & Shipping

• Store at - 20 °C

• The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.
  

Documents

Yersinia enterocolitica Probe qPCR Kit User Manual

MSDS