Torque teno Virus Probe qPCR Kit

Description

Torque teno virus (TTV) is a small, single-stranded circular DNA virus widely present in the human virome, with detection rates exceeding 90% in adults. Although not associated with a specific disease, TTV exhibits a strong correlation with host immune activity: viral loads tend to rise during immunosuppression and decrease as immune pressure increases. This unique behavior has led to growing interest in TTV as an endogenous biomarker for monitoring immune function and viral kinetics. The Torque teno Virus Probe qPCR Kit enables quantitative detection of TTV-specific DNA using hydrolysis probe–based real-time PCR. During amplification, the 5' nuclease activity of Taq DNA polymerase cleaves the hybridized probe, releasing the quenched FAM fluorophore. As fluorescence increases, the system measures the accumulation of TTV-specific amplicons and assigns a cycle threshold (Ct) value once the signal rises above background. This Ct value provides a reliable measure of TTV DNA abundance, supporting precise viral load analysis for immunology, transplant immunosuppression, and virome research.

Key Features

One-tube qPCR format minimizes carry-over contamination risk

• High sensitivity: analytical LOD as low as 100 copies/reaction

• Excellent specificity: no cross-reactivity with DNA from other pathogens

• Includes amplification positive control for result verification

• Compatible with various DNA purification methods

• Suitable for both qualitative and quantitative applications

• For quantitative use: the linear range covers a 100,000-fold concentration difference.

• Sufficient for 50 reactions (20 μL each)

• For Research Use Only (RUO)

Kit Components

Application Areas

• Transplant Immunology Research

  Monitor TTV viral load in kidney, liver, heart, and lung transplant cohorts to evaluate net immunosuppression, identify rejection or infection risk trends, and support studies on TTV-guided immunosuppressive optimization.

• Immune Function & Virome Studies

  Quantify TTV as an endogenous biomarker of immune activity for research on immune suppression, immune recovery, viral kinetics, chronic inflammation, and host–virome interactions.

• HSCT, CAR-T & Immunotherapy Research

  Track TTV dynamics to assess T-cell recovery, delayed immune reconstitution, and infection susceptibility in allo-HSCT, CAR-T, and other immune-modulating therapy models.

• HIV & Immunodeficiency Cohorts

  Measure TTV levels to study immune restoration during antiretroviral therapy and evaluate immune competence in immunodeficient populations.

• Blood Product & Transfusion Research

  Analyze TTV in donor blood and plasma for studies on virome composition, viral persistence, and transfusion-related immune modulation (RUO).

• Veterinary & Comparative Virology

  Detect and quantify torque teno viruses across animal species to support research on host range, viral diversity, and cross-species virome ecology.

Storage & Shipping

• Store at - 20 °C

• The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.
  

FAQ

Q1: Are Biofargo PCR and qPCR Kits compatible with my thermal cycler or real-time PCR instrument?

A: Yes. Biofargo PCR Kits and probe-based qPCR Kits are designed for broad compatibility with mainstream instruments.

PCR Kits can be used with standard thermal cyclers from leading brands.

Probe-based qPCR Kits are fully compatible with major real-time PCR platforms, including ABI, Bio-Rad, Roche, QuantStudio, Qiagen Rotor-Gene Q (5-plex), and Genesig Q16.

Our technical team is available to assist with instrument setup and run optimization to ensure the best results.

Q2: Can this kit be used for digital droplet PCR (ddPCR)?

A: Yes.

Q: Does this product contain sodium azide, thimerosal, 2-mercaptoethanol, or any other toxic preservatives?

A: cetonitrile (> 99.9%) forHPLC or LC-MS Certified ACS Acetone Glacial Acetic Acid Imidazole (buffer) Methanol 99.8% ACS.

Documents

Torque teno Virus Probe qPCR Kit User Manual

MSDS