Skygen Clostridium hastiforme Probe Realtime PCR Kit (Without Internal Control)

Description

Skygen Clostridium hastiforme Probe Realtime PCR Kit (Without Internal Control) is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5’ nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.

Features

• One-tube PCR，minimized carry-over contamination.

• High sensitivity，with an analytical LOD at 100 copies/reaction.

• High specificity，no cross-reaction with DNA from other pathogens.

• Amplification positive control is provided in the kit.

• Compatible with various DNA purification methods.

• Suitable for both qualitative and quantitative detection. For quantitative use, the linear range is at least 5 orders of magnitude.

• Enough for 50 reactions，20 μL per reaction.

• Research use only.