Clostridium difficile Probe qPCR Kit

All-in-One qPCR Kit for Rapid and Precise Clostridium difficile Detection in Clinical Diagnostics – 50 Reactions

$456.00

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Sku: 15-34530
Categories: Cell & Molecular Biology , Custom Solutions , Pathogen Detection Kits , qPCR Kit
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Description

Clostridium difficile Probe qPCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.

Key Features

One-tube qPCR format minimizes carry-over contamination risk

  • High sensitivity: analytical LOD as low as 100 copies/reaction

  • Excellent specificity: no cross-reactivity with DNA from other pathogens

  • Includes amplification positive control for result verification

  • Instrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc.

  • Suitable for both qualitative and quantitative applications

  • For quantitative use: the linear range covers a 100,000-fold concentration difference.

  • Sufficient for 50 reactions (20 μL each)

  • For Research Use Only (RUO)

Kit Components

Component Name Product Number. Size Vial Lid Color
2×Probe qPCR MasterMix 981201 0.5 mL 0.5 mL Vial Natural
Template Dilution Buffer 180701 1 mL 1.5 mL Vial Green
Primer-Probe Powder yp15-34530 50 T 0.5 mL Brown Vial Brown
Amplification Positive Control
(1E7 copies/μL)		 pc34530 50 μL 0.5 mL Vial Yellow
Ultrapure water 210806 1 mL 1.5 mL Vial Blue
Manual sc15-34530 1 Set NA NA

 

Application Areas

  • Aquaculture Biosecurity & Shellfish Health: high-throughput qPCR screening of C. difficile in farmed shellfish and marine organisms for optimized stock management and disease prevention
  • Clinical Microbiology & Antimicrobial Research: precise quantification of gut microbiota dysbiosis and evaluation of antibiotic efficacy in preclinical and translational studies
  • Seafood Safety & Marine Environmental Surveillance: sensitive detection of C. difficile in seafood processing, distribution chains, and coastal waters to ensure product quality and ecosystem health

Storage & Shipping

  • Store at - 20 °C

  • The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.

Documents

Clostridium difficile Probe qPCR Kit User Manual

MSDS

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

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