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$470.00
Sku: BF-51890847
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by April 08, 2026
Description
Bradyrhizobium japonicum Probe qPCR Kit is a PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified DNA is directly amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification. The resulting signals may be used for comparative analysis of target nucleic acids.
Note: Supplied without internal control. Internal control version can be customized based on your assay requirements.
This product was previously listed under catalog number 15-44805 and is now updated to BF-51890847 for improved product management.
Key Features
Streamlined PCR workflow to reduce handling steps and minimize contamination risk
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Demonstrates high sensitivity under optimized laboratory conditions.
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High specificity based on primer-probe design.
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Includes a non-infectious plasmid-based positive control for assay verification.
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Compatible with commonly used DNA purification methods.
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Suitable for research applications including assay development and method evaluation.
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Broad dynamic range under laboratory conditions.
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For research use only. Not intended for diagnostic or therapeutic use.
Kit Components
| Component Name | Description | Size | Vial | Lid Color |
| 2×Probe qPCR Master Mix | Contains optimized buffer and enzyme mix | 500 μL | 0.5 mL Vial | Blue |
| Primer-Probe Powder | Lyophilized target-specific primer and probe set | 50 T | 1.5 mL Vial | Brown |
| Positive Control (1E7 copies/μL) | Non-infectious plasmid-based control | 0.5 mL | 0.5 mL Brown Vial | Yellow |
| Nuclease-free water / Dilution Buffer | For reaction setup | 1 mL | 1.5 mL Vial | Green |
| Manual | Instruction booklet | 1 Set | NA | NA |
Application Areas
- Molecular biology research: qPCR-based detection and quantification of target nucleic acids.
- Assay development: Suitable for method development, optimization, and analytical performance evaluation.
- Industry applications (research use): Widely used in biotechnology, pharmaceutical, academic, and agricultural research laboratories.
- For Research Use Only (RUO). Not for use in diagnostic procedures.
Storage & Shipping
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Store at - 20 °C
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The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.
FAQ
Q: Are Biofargo PCR and qPCR Kits compatible with my thermal cycler or real-time PCR instrument?
A: Yes. Biofargo PCR Kits and probe-based qPCR Kits are designed for broad compatibility with mainstream instruments.
PCR Kits can be used with standard thermal cyclers from leading brands.
Probe-based qPCR Kits are fully compatible with major real-time PCR platforms, including ABI, Bio-Rad, Roche, QuantStudio, Qiagen Rotor-Gene Q (5-plex), and Genesig Q16.
Our technical team is available to assist with instrument setup and run optimization to ensure the best results.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
