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$61.00
Sku: AP0761
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by July 15, 2026
Description
SDS Lysis Buffer is a strong denaturing protein extraction buffer designed for efficient cell and tissue lysis when mild lysis reagents cannot fully release target proteins. During protein sample preparation, incomplete disruption of cellular structures, poor solubilization of membrane-associated proteins, or insufficient extraction efficiency from complex biological samples can affect downstream protein analysis. This SDS-based cell lysis buffer provides powerful protein solubilization capability to generate reliable protein lysates for Western blot, SDS-PAGE, and other protein analysis workflows.
Formulated with SDS detergent, Tris buffer, EDTA, and protein protection components, SDS Lysis Buffer enables rapid disruption of cellular membranes and efficient extraction of total proteins from animal cells, plant samples, bacteria, yeast, and tissue specimens. Compared with mild non-denaturing lysis buffers, this strong protein extraction buffer provides enhanced recovery of difficult-to-solubilize proteins and is particularly suitable for applications requiring complete protein denaturation and maximum protein yield.
SDS Lysis Buffer is optimized for preparing protein lysates for electrophoretic and antibody-based detection workflows. Due to the presence of SDS detergent, lysates prepared with this buffer are not suitable for Bradford protein assays and should be quantified using detergent-compatible protein determination methods when required.
Specifications
| Product Name | SDS Lysis Buffer |
| SKU | AP0761 |
| Volume | 100 mL |
| Product Type | Strong SDS Protein Extraction Buffer |
| Buffer Composition | 50 mM Tris (pH 8.1), 1% SDS, EDTA, protease and phosphatase inhibitor components |
| Applications | Protein extraction, Western Blot, SDS-PAGE, ELISA, IP, ChIP sample preparation |
| Sample Types | Animal cells, tissues, plants, bacteria, yeast |
| Storage | -20 °C |
| Shelf Life | 18 months |
Features
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Strong protein extraction capability: Provides efficient cell disruption and protein solubilization for samples containing difficult-to-extract proteins.
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SDS-based denaturing lysis system: Uses SDS detergent to disrupt cellular membranes and promote complete protein extraction.
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Efficient cell and tissue lysis: Suitable for preparation of whole-cell lysates and tissue protein extracts from diverse biological samples.
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Enhanced recovery of membrane-associated proteins: Supports extraction of proteins tightly associated with cellular structures.
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Protein degradation protection: Contains EDTA and inhibitor components to help maintain protein integrity during sample preparation.
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Compatible with electrophoresis workflows: Ideal for SDS-PAGE and Western blot protein sample preparation.
Applications
SDS Lysis Buffer is widely used as a strong protein extraction reagent in molecular biology, biochemical research, and protein analysis workflows where efficient solubilization and high protein recovery are required.
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Western Blot Protein Extraction: Generates high-quality protein lysates for SDS-PAGE separation and Western blot detection of target proteins.
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SDS-PAGE Sample Preparation: Provides denatured protein samples suitable for electrophoretic protein separation and analysis.
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Total Protein Extraction: Enables efficient extraction of total cellular proteins from cultured cells and biological samples.
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Cell Protein Extraction: Suitable for adherent cells and suspension cells requiring strong lysis conditions.
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Tissue Protein Extraction: Supports extraction of proteins from animal and plant tissues where stronger disruption is required.
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Membrane Protein Extraction: Helps improve solubilization of membrane-associated and difficult-to-extract proteins.
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Bacterial and Yeast Protein Extraction: Can be used for microbial protein preparation with optional enzymatic pretreatment when additional disruption is required.
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ChIP Sample Preparation: Suitable for chromatin-related sample preparation workflows when SDS-compatible downstream processing is applied.
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ELISA and Protein Analysis: Provides prepared protein lysates for selected immunoassay and biochemical analysis workflows.
Protocol Overview
For optimal protein extraction performance, samples should be processed under cold conditions to minimize protein degradation.
- Add PMSF or other required protease/phosphatase inhibitors before use according to experimental requirements.
- Lyse samples on ice or at 4 °C to maintain protein stability.
- Add SDS Lysis Buffer to cells or tissues and ensure complete sample disruption.
- Centrifuge lysates at 10,000–14,000 ×g for 3–5 minutes and collect the clarified supernatant for downstream analysis.
Because SDS-containing lysates contain high detergent concentrations, Bradford protein assays are not recommended. Detergent-compatible protein quantification methods should be selected when protein concentration measurement is required.
Ordering Information
| SKU | Product | Size |
| AP0761 | SDS Lysis Buffer | 100 mL |
FAQ
What is SDS Lysis Buffer used for?
SDS Lysis Buffer is a strong cell lysis and protein extraction reagent used to prepare protein lysates from cells, tissues, bacteria, yeast, and plant samples. It is commonly used for Western blot, SDS-PAGE, and total protein extraction workflows.
Is SDS Lysis Buffer suitable for Western blot?
Yes. SDS Lysis Buffer is especially suitable for Western blot sample preparation because SDS efficiently solubilizes proteins and produces denatured protein lysates compatible with SDS-PAGE analysis.
What is the difference between SDS Lysis Buffer and RIPA Buffer?
SDS Lysis Buffer provides stronger denaturing extraction through SDS detergent and is designed for maximum protein solubilization. RIPA buffers are generally milder and are often selected when partial preservation of protein complexes or native protein structures is required.
Can SDS Lysis Buffer be used for protein interaction studies?
SDS Lysis Buffer is a strong denaturing lysis reagent and may disrupt many protein-protein interactions. For native protein interaction studies such as Co-IP, milder non-denaturing buffers are generally preferred.
Can Bradford assay be used after SDS Lysis Buffer extraction?
No. SDS detergent can interfere with Bradford protein quantification and may result in inaccurate measurements. Detergent-compatible protein assays should be considered instead.
What samples can be extracted using SDS Lysis Buffer?
SDS Lysis Buffer can be used for animal cells, tissue samples, plant materials, bacteria, and yeast. Sample disruption conditions may require optimization depending on sample characteristics.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
