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Sku: FS-M2005
S96 96-Well Fluorometer FS-M2005——StarLighter
Description
Laboratories processing multiple DNA, RNA, microRNA, or protein samples may need to repeat individual measurements when using a single-sample instrument. The StarLighter Fluorometer S96 FS-M2005 provides a 96-well fluorescence measurement format for quantitative analysis of up to 96 sample positions. This high-throughput nucleic acid quantification fluorometer requires only 1–20 µL of sample and completes a whole-plate measurement in 55 seconds.
The FS-M2005 operates as a two-channel, top-reading fluorescence microplate reader. It uses LED excitation and an SiPMT detector to measure dsDNA, ssDNA, RNA, microRNA, and protein assays. Blue excitation is provided at 457–483 nm with green emission detection at 520–540 nm, while red excitation is provided at 622–638 nm with red emission detection at 672–712 nm.
The instrument uses a 0.3 mL 96-well flat-bottom black ELISA plate measuring 128 × 86 × 15 mm. Sample volume can be set between 1 and 20 µL, and the combined sample and working-solution volume must be 200 µL per well. Experiment protocols, well positions, standards, blanks, sample information, and measurement results are managed through the supplied PC software. Results can be saved and exported in Excel format.
This 96-well fluorometer is for research use only and is not intended for diagnostic or therapeutic procedures.
Specification
| Product Name | StarLighter Fluorometer S96 |
|---|---|
| Product Model | FS-M2005 |
| Instrument Type | 96-well fluorometer |
| Sample Throughput | 96 positions |
| Sample Volume | 1–20 µL |
| Total Reaction Volume | 200 µL per well |
| Compatible Plate | 0.3 mL 96-well flat-bottom black ELISA plate |
| Compatible Plate Dimensions | 128 × 86 × 15 mm; consumable height must not exceed 15 mm |
| Whole-Plate Test Time | 55 seconds |
| Light Source | LED |
| Fluorescence Channels | 2 |
| Excitation Wavelengths | Blue: 457–483 nm; Red: 622–638 nm |
| Emission Wavelengths | Green: 520–540 nm; Red: 672–712 nm |
| Detector | SiPMT |
| Detection Method | Top detection |
| Supported Sample Types | dsDNA, ssDNA, RNA, microRNA, and proteins |
| Sensitivity | dsDNA: 0.05 ng/µL; ssDNA: 0.1 ng/µL; RNA: 0.5 ng/µL; microRNA: 0.1 ng/µL |
| DNA HS Detection Range | 0.01–100 ng/µL |
| DNA BR Detection Range | 1–1000 ng/µL |
| RNA Detection Range | 0.25–100 ng/µL |
| RNA BR Detection Range | 1–1000 ng/µL |
| Calibration | 2-point calibration |
| Dynamic Range | 5 orders of magnitude |
| Communication Interface | Bluetooth and serial port |
| Software Languages | Chinese and English |
| Software Temperature Setting | Ambient temperature to 37°C |
| Power | DC 24 V, 2.5 A; 60 W maximum |
| Operating Environment | 15–30°C; less than 75% RH; indoor use only |
| Transport and Storage Temperature | −20–60°C |
| Operating Altitude | Up to 2,000 m above sea level |
| Dimensions | 274 × 180 × 106 mm |
| Weight | 2.7 kg |
| Intended Use | For research use only |
Features
- 96-well sample throughput: The instrument provides 96 sample positions for high-throughput fluorescence measurement.
- Low sample-volume requirement: Each measurement uses 1–20 µL of sample.
- 55-second whole-plate reading: A complete plate can be measured in 55 seconds.
- Two fluorescence channels: The optical system provides blue and red excitation channels with green and red emission detection.
- Multiple supported analytes: Test items include dsDNA, ssDNA, RNA, microRNA, proteins, oligo, blue-light scanning, and red-light scanning.
- SiPMT top-reading detection: Fluorescence is measured from the top of the 96-well plate using an SiPMT detector.
- Flexible well configuration: The software supports SAMPLE, STANDARD, BLANK, and RFU well types.
- Protocol management: Users can create, save, and delete experimental protocols and modify sample information and well positions.
- Result management: Experimental data can be imported, saved, deleted, annotated, and exported in Excel format.
- Chinese and English software: The PC software interface supports Chinese and English language selection.
Package Contents
| Item | Quantity |
|---|---|
| Fluorescence Reader | 1 unit |
| Power cord | 1 piece |
| USB flash drive containing the user manual, communication protocol, and software installation package | 1 piece |
| Packing list | 1 copy |
| USB cable | 1 piece |
| Power adapter | 1 piece |
| Black ELISA plate | 1 piece |
Recommended Assay Kits
The user guide lists the following StarLighter and Thermo Fisher assay kits for use with the 96-well fluorometer.
| Assay Kit | Manufacturer | Catalog Number |
|---|---|---|
| StarLighter dsDNA HS Assay Kit | Beijing ForeverStar Biotech | FS-T1002 |
| StarLighter dsDNA BR Assay Kit | Beijing ForeverStar Biotech | FS-T1004 |
| StarLighter 1× dsDNA HS Assay Kit | Beijing ForeverStar Biotech | FS-T1005 |
| StarLighter RNA HS Assay Kit | Beijing ForeverStar Biotech | FS-T1006 |
| StarLighter RNA BR Assay Kit | Beijing ForeverStar Biotech | FS-T1007 |
| StarLighter ssDNA Assay Kit-V2 | Beijing ForeverStar Biotech | FS-T1008-V2 |
| dsDNA HS Assay Kit | Thermo Fisher | Q32851 |
| Qubit dsDNA BR Assay Kit | Thermo Fisher | Q32850 |
| Qubit ssDNA Assay Kit | Thermo Fisher | Q10212 |
| Qubit RNA HS Assay Kit | Thermo Fisher | Q32852 |
| Qubit Protein BR Assay Kit | Thermo Fisher | A50668 / A50669 |
| Qubit RNA BR Assay Kit | Thermo Fisher | Q10210 |
| Qubit RNA XR Assay Kit | Thermo Fisher | Q33223 |
Application
The StarLighter S96 is designed for fluorescence-based quantitative detection using a 96-well flat-bottom black microplate. Supported test applications stated in the user guide include:
- dsDNA quantitative detection
- ssDNA quantitative detection
- RNA quantitative detection
- microRNA quantitative detection
- Protein quantitative detection
- Oligo measurement
- Blue-light fluorescence scanning
- Red-light fluorescence scanning
- RFU fluorescence-value measurement
The instrument is intended for indoor laboratory research use only. It is not intended for diagnostic or therapeutic procedures.
FAQ
What is the StarLighter S96 FS-M2005?
The StarLighter S96 FS-M2005 is a 96-well fluorometer designed for quantitative fluorescence detection of dsDNA, ssDNA, RNA, microRNA, and proteins. It uses two fluorescence channels, LED excitation, top detection, and an SiPMT detector.
How many samples can the FS-M2005 measure per run?
The instrument provides 96 sample positions and completes a whole-plate measurement in 55 seconds.
What sample volume and microplate are required?
The sample volume can be set from 1 to 20 µL. The combined sample and working-solution volume must be 200 µL per well. The instrument uses a 0.3 mL 96-well flat-bottom black ELISA plate measuring 128 × 86 × 15 mm, with a maximum consumable height of 15 mm.
What are the sensitivity and detection ranges?
The specified sensitivities are 0.05 ng/µL for dsDNA, 0.1 ng/µL for ssDNA, 0.5 ng/µL for RNA, and 0.1 ng/µL for microRNA. The detection ranges are 0.01–100 ng/µL for DNA HS, 1–1000 ng/µL for DNA BR, 0.25–100 ng/µL for RNA, and 1–1000 ng/µL for RNA BR.
What fluorescence wavelengths does the instrument use?
The blue excitation channel covers 457–483 nm with green emission detection at 520–540 nm. The red excitation channel covers 622–638 nm with red emission detection at 672–712 nm.
Which assay kits are listed for use with the fluorometer?
The user guide lists StarLighter dsDNA HS, dsDNA BR, 1× dsDNA HS, RNA HS, RNA BR, and ssDNA Assay Kit-V2 products. It also lists specific Thermo Fisher dsDNA, ssDNA, RNA, and Protein assay kits with their catalog numbers.
Can the results be exported to Excel?
Yes. The PC software supports protocol management, standard-curve calculations, result storage, experiment notes, historical-data import, and Excel-format data export.
Is the StarLighter S96 intended for diagnostic use?
No. The StarLighter S96 FS-M2005 is for research use only and is not intended for diagnostic or therapeutic procedures.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
