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Note:Suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and hybridomas, require supplementation, commonly with 10% Fetal Bovine Serum (FBS).
Product Description
RPMI-1640 Medium was developed by Moore et al., at Roswell Park Memorial Institute. It was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI-1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and hybridomas. RPMI-1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).
Specifications
| Volume | 500mL |
| Supplementation | Need additional |
| Glutamine | L-Glutamine |
| Phenol Red | Phenol Red |
| Antibiotics | No Antibiotics |
| Sodium Pyruvate | No Sodium Pyruvate |
| Sodium Bicarbonate | Sodium Bicarbonate |
| HEPES Buffer | No HEPES |
| pH | 7.0-7.6 |
| Osmolality | 265~305 mOsmol/kg |
| Endotoxin | <0.3 EU/mL |
Formulation
Components (mg/L)
Amino Acids
| Glycine | 10 |
| L-Arginine (free base) | 200 |
| L-Asparagine (anhyd.) | 50 |
| L-Aspartic Acid | 20 |
| L-Cystine•2HCl | 65 |
| L-Glutamic Acid | 20 |
| L-Glutamine | 300 |
| L-Histidine (free base) | 15 |
| L-Hydroxyproline | 20 |
| L-Isoleucine | 50 |
| L-Leucine | 50 |
| L-Lysine•HCl | 40 |
| L-Methionine | 15 |
| L-Phenylalanine | 15 |
| L-Proline | 20 |
| L-Serine | 30 |
| L-Threonine | 20 |
| L-Tryptophan | 5 |
| L-Tyrosine•2Na•2H₂O | 29 |
| L-Valine | 20 |
Vitamins
| D-Biotin | 0.2 |
| Choline chloride | 3 |
| Folic Acid | 1 |
| myo-Inositol | 35 |
| Niacinamide | 1 |
| p-Amino Benzoic Acid | 1 |
| D-Pantothenic Acid•1/2 Ca | 0.25 |
| Pyridoxal•HCl | 1 |
| Riboflavin | 0.2 |
| Thiamine•HCl | 1 |
| Vitamin B-12 | 0.005 |
Inorganic Salts
| Ca(NO₃)₂•4H₂O | 100 |
| MgSO₄(anhyd.) | 48.84 |
| KCl | 400 |
| NaHCO₃ | 2000 |
| NaCl | 6000 |
| NaH₂PO₄(anhyd.) | 800 |
Other
| D-Glucose | 2000 |
| Glutathione (reduced) | 1 |
| Phenol Red•Na | 5 |
Storage
Stored at 2℃~8℃, away from light, the shelf life is 12 months.
Notes
Avoid repeated freezing and thawing.
Pay attention to operate in an aseptic condition.
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