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Sku: 6016011
Categories: Medium

Note:Suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and hybridomas, require supplementation, commonly with 10% Fetal Bovine Serum (FBS).

 

Product Description

RPMI-1640 Medium was developed by Moore et al., at Roswell Park Memorial Institute. It was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI-1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and hybridomas. RPMI-1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

 

Specifications

Volume 500mL
Supplementation Need additional
Glutamine L-Glutamine
Phenol Red Phenol Red
Antibiotics No Antibiotics
Sodium Pyruvate No Sodium Pyruvate
Sodium Bicarbonate Sodium Bicarbonate
HEPES Buffer No HEPES
pH 7.0-7.6
Osmolality 265~305 mOsmol/kg
Endotoxin <0.3 EU/mL

 

 

Formulation

Components (mg/L)

Amino Acids

Glycine 10
L-Arginine (free base) 200
L-Asparagine (anhyd.) 50
L-Aspartic Acid 20
L-Cystine•2HCl 65
L-Glutamic Acid 20
L-Glutamine 300
L-Histidine (free base) 15
L-Hydroxyproline 20
L-Isoleucine 50
L-Leucine 50
L-Lysine•HCl 40
L-Methionine 15
L-Phenylalanine 15
L-Proline 20
L-Serine 30
L-Threonine 20
L-Tryptophan 5
L-Tyrosine•2Na•2H₂O 29
L-Valine 20

 

Vitamins

D-Biotin 0.2
Choline chloride 3
Folic Acid 1
myo-Inositol 35
Niacinamide 1
p-Amino Benzoic Acid 1
D-Pantothenic Acid•1/2 Ca 0.25
Pyridoxal•HCl 1
Riboflavin 0.2
Thiamine•HCl 1
Vitamin B-12 0.005

 

Inorganic Salts

Ca(NO₃)₂•4H₂O 100
MgSO₄(anhyd.) 48.84
KCl 400
NaHCO₃ 2000
NaCl 6000
NaH₂PO₄(anhyd.) 800

 

Other

D-Glucose 2000
Glutathione (reduced) 1
Phenol Red•Na 5

 

Storage

Stored at 2℃~8℃, away from light, the shelf life is 12 months.

 

Notes

Avoid repeated freezing and thawing.
Pay attention to operate in an aseptic condition.

 

Document

RPMI-1640 Medium-Biosci™

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