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$97.60
Sku: SIN-201
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by June 13, 2026
Description
This product is a recombinant type human ribonuclease inhibitor purified using a combination of ion exchange and affinity chromatography.
This inhibitor exhibits broad-spectrum RNase inhibitory properties, including RNase A, RNase B, RNase C and human placental RNase. Does not inhibit RNase T1, S1 nuclease, RNase from Aspergillus, and RNase H. The 50kDa protein exerts its inhibitory effect by noncovalently binding to RNases in a 1:1 ratio. This inhibitor can be applied to a reverse transcriptase reaction.
Features
- • Stabilizes RNA in a cDNA synthesis or a RNA synthesis reaction.
- • Dees not inhibit SP6, T7 or T3 RNA Polymerase, AMV or M-MLV Reverse Transcriptase, or Taq DNA polymerase.
- • Exhibits broad-spectrum RNase inhibitory properties.
- • Active over a broad pH range (pH 5-8).
Application
cDNA synthesis, in vitro transcription, in vitro translation
Specification
| Category | Details |
|---|---|
| Source | E. coli strain carrying the cloned human placenta ribonuclease inhibitor gene |
| Unit definition | One unit is defined as the amount of RNase inhibitor required to inhibit the activity of 5 ng of ribonuclease A by 50%. |
| Storage condition | 20 mM Hepes-KOH (pH 7.6), 50 mM KCl, 8 mM DTT, 50% Glycerol Store at -20ºC |
| Components | RNase inhibitor (20-40 U/µL)* |
| Specific activity: approximately 100 U/µg protein |
Application Data
Example 1.Inhibition effect of RNase inhibitor on RT-PCR
Reverse transcription was performed with a random primer using total RNA from HeLa cells as a template with or without RNase inhibitor and RNase A. The results of amplification of human IL-8 genes from the cDNA suggest that the addition of RNase inhibitor to the reverse transcription step effectively and efficiently protects mRNA.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
