RNAlater (For RNA Stabilization)

Descprition

Once the biological sample is collected, its RNA becomes quite unstable. Rapidly stabilizing RNA and maintaining RNA expression are the first conditions for obtaining accurate gene expression analysis data. In addition, it is also necessary to prevent the activation of increased or down-regulated gene expression caused by sample processing.

RNAlater is a liquid non-toxic animal tissue preservation reagent. It can quickly penetrate into tissue cells and protect non-frozen cellular RNA from degradation by effectively inhibiting RNase activity, so that after the tissue sample is obtained, it is not necessary to process the sample immediately, and it is not necessary to freeze the sample in liquid nitrogen,so it is more convenient for subsequent experimental operations. The use of tissue RNA protection solution can avoid the inconvenience of using liquid nitrogen or ultra-low temperature refrigerators, and storing different batches of tissue specimens in the protection solution can immediately stop and fix the sequence changes of RNA expression, which can reduce the error between experimental groups .

RNAlater can be widely used in a variety of vertebrate samples, including brain, heart, kidney, spleen, liver, and lung. After fresh non-frozen tissues are immersed in Buffer RNAlater at a ratio of 1:10, the samples can be stored at room temperature for 1 week, 37°C for 1 day, 4°C for at least 1 month; tissues can be immersed at 4°C at -20°C or store at -80℃ for a long time.

Features

• Reagents are used directly, one step is in place, and RNA is immediately stabilized and protected.

• Operation at room temperature, convenient, safe and non-toxic.

• To get rid of the trouble of dry ice or low-temperature refrigerator for tissue preservation.

• The tissue can be stored for a long time without the risk of RNA degradation, ensuring reliable gene expression profile data.

Application

It is used to immediately preserve freshly collected animal tissues and immediately stabilize the RNA in the tissue samples to preserve gene expression profiles.

Storage

It can be stored stably for more than 1 year at room temperature (15-25℃).

Note: Precipitation or crystal precipitation may occur when stored at low temperatures. The precipitate must be completely dissolved at room temperature or 37°C before use.