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Sku: FS-T1007-S
RNA BR Assay Kit for Broad Range RNA Quantification——StarLighter
Description
Laboratories measuring RNA across a broad concentration range need an assay that selectively detects RNA while accommodating different sample volumes and common laboratory contaminants. The StarLighter RNA BR Assay Kit provides a fluorescence-based method for broad range RNA quantification. It is highly selective for RNA over double-stranded DNA (dsDNA) and detects initial RNA concentrations from 1 ng/µL to 100 ng/µL, depending on sample volume, with a total assay range of 20–1000 ng.
This RNA Broad Range Assay Kit accepts 1–20 µL of sample in a final assay volume of 200 µL. The protocol can be performed with either a Qubit fluorometer or a fluorescence microplate reader. For microplate-based RNA quantification, fluorescence is measured at 620 nm excitation and 660 nm emission.
The assay tolerates common contaminants, including salts, free nucleotides, solvents, detergents, proteins, agarose, and PEG at the concentrations listed below. Signal development requires a 2-minute incubation at room temperature while protected from light. When protected from light, the fluorescent signal remains stable for up to 3 hours after incubation.
Specification
| Product Name | StarLighter RNA BR Assay Kit |
|---|---|
| Product Type | RNA Broad Range Assay Kit |
| Catalog Numbers | FS-T1007-S; FS-T1007 |
| Initial RNA Concentration Range | 1–100 ng/µL, depending on sample volume |
| Total Assay Range | 20–1000 ng |
| Sample Volume | 1–20 µL |
| Final Assay Volume | 200 µL |
| Selectivity | Highly selective for RNA over double-stranded DNA (dsDNA) |
| Compatible Instruments | Qubit fluorometer or fluorescence microplate reader |
| Microplate Reader Format | 96-well format |
| Excitation | 620 nm |
| Emission | 660 nm |
| Assay Temperature | Room temperature, 20–25°C |
| Incubation | 2 minutes at room temperature, protected from light |
| Signal Stability | Up to 3 hours after incubation when protected from light |
| Shipping Condition | Ship on ice |
| Standards Storage | Store QS0 and QS1 at -25 to -15°C |
| Other Components Storage | Store at 2–8°C |
| Intended Use | For Research Use Only. Not for use in diagnostic or therapeutic procedures. |
Kit Components
| Component | FS-T1007-S (100 Reactions) | FS-T1007 (1000 Reactions) |
|---|---|---|
| RNA BR Buffer QB | 20 mL | 200 mL |
| RNA BR Standard QS0 | 100 µL, 0 ng/µL | 1 mL, 0 ng/µL |
| RNA BR Standard QS1 | 100 µL, 100 ng/µL | 1 mL (4 × 250 µL), 100 ng/µL |
| RNA BR Fluorescent Reagent QF | 100 µL | 1 mL |
Required Materials Not Supplied
- Qubit fluorometer or fluorescence microplate reader
- Thin-walled, clear 0.5 mL PCR tubes for the Qubit fluorometer
- Black 96-well microplate for the microplate reader
- Vortex mixer
- Pipettes and sterile, RNase-free pipette tips
Contaminant Tolerance
| Contaminant | Final Concentration in Assay | Concentration in 10 µL Sample | Result |
|---|---|---|---|
| Protein (BSA) | 10 mg/mL | 200 mg/mL | OK |
| Sodium acetate | 20 mM | 400 mM | OK |
| Ammonium acetate | 20 mM | 400 mM | OK |
| NaCl | 20 mM | 400 mM | OK |
| MgCl2 | 5 mM | 100 mM | OK |
| Phenol | 0.1% | 2% | OK |
| Chloroform | 0.5% | 10% | OK |
| Ethanol | 0.5% | 10% | OK |
| SDS | 0.01% | 0.2% | OK |
| Triton X-100 | 0.01% | 0.2% | OK |
| Agarose | 0.1% | 2% | OK |
| PEG | 1% | 20% | OK |
| dNTPs | 100 µM | 2 mM | OK |
The listed sample concentrations are approximate equivalents for a 10 µL sample volume. An “OK” result usually represents less than 10% perturbation under the stated assay conditions.
Features
- Fluorescent RNA quantification across a total assay range of 20–1000 ng
- Initial RNA concentration range of 1–100 ng/µL, depending on sample volume
- High selectivity for RNA over double-stranded DNA
- Flexible sample input from 1–20 µL
- Compatible with a Qubit fluorometer or fluorescence microplate reader
- Two-minute signal development at room temperature
- Fluorescent signal remains stable for up to 3 hours when protected from light
- Tolerates the listed salts, solvents, detergents, proteins, agarose, PEG, and dNTPs
- Available in 100-reaction and 1000-reaction configurations
Application
The StarLighter RNA BR Assay Kit is intended for broad range RNA quantification in research laboratories. It supports fluorescence-based RNA measurement using either individual assay tubes with a Qubit fluorometer or a black 96-well microplate with a fluorescence microplate reader.
- Broad range RNA quantification from 20–1000 ng total RNA
- RNA concentration measurement from samples containing 1–100 ng/µL RNA
- Tube-based RNA quantification with a Qubit fluorometer
- 96-well fluorescence microplate-based RNA quantification
This product is for research use only and is not intended for diagnostic or therapeutic procedures.
FAQ
What is the quantification range of the StarLighter RNA BR Assay Kit?
The assay detects initial RNA concentrations from 1 ng/µL to 100 ng/µL, depending on the sample volume. Its total assay range is 20–1000 ng.
How much RNA sample is required for each assay?
Each assay accepts 1–20 µL of RNA sample. The working solution volume must be adjusted so that the final assay volume is exactly 200 µL.
Can this RNA Broad Range Assay Kit be used with a Qubit fluorometer?
Yes. The protocol is compatible with a Qubit fluorometer. The assay requires two standards, QS0 and QS1, and thin-walled, clear 0.5 mL PCR tubes. On a Qubit 4 Fluorometer, RNA Broad Range is selected as the assay type.
Can the kit be used with a fluorescence microplate reader?
Yes. The assay supports a 96-well fluorescence microplate reader workflow. Use a black-wall, clear-bottom microplate to minimize cross-talk and set the reader to 620 nm excitation and 660 nm emission.
How long should the RNA assay incubate before measurement?
Incubate the prepared tubes or microplate for 2 minutes at room temperature, 20–25°C, while protected from light. The fluorescent signal remains stable for up to 3 hours after incubation when protected from light.
Is the assay selective for RNA over DNA?
Yes. The StarLighter RNA BR Assay is highly selective for RNA over double-stranded DNA.
Which StarLighter RNA BR Assay Kit sizes are available?
Catalog number FS-T1007-S contains materials for 100 reactions. Catalog number FS-T1007 contains materials for 1000 reactions.
How should the StarLighter RNA BR Assay Kit be stored?
The kit ships on ice. RNA BR Standards QS0 and QS1 must be stored at -25 to -15°C. All other kit components should be stored at 2–8°C. The fluorescent reagent, working solution, and assay tubes must be protected from light to minimize photobleaching.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
