RFP Expressing Rat Primary Lymphatic Endothelial Cells

Description

RFP-Expressing Rat Primary Lymphatic Endothelial Cells from Cell Biologics are isolated from the lymph nodes of Sprague-Dawley rats and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10⁶ cells per ml and is delivered frozen. RFP-Expressing Rat Primary Lymphatic Endothelial Cells are characterized by use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.

Red Fluorescent Protein (RFP), a mutant form of green fluorescent protein, gives rise to very bright red fluorescence when exposed to yellow-green light. Cell Biologics generates various RFP-expressing stable cells, including endothelial cells, epithelial cells and so forth. RFP-expressing cells are developed through transducing RFP-lentiviral particles.

Suggested Medium

M1266 Complete Endothelial Cell Medium w/ Kit – 500 ml

Storage

Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Documents

RA-6092RFP

Cell-Culture-Protocol-CB

1708462493

1698246847

Disclaimer

Investigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.