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Description
SHENTEK® Residual Vero DNA Size Analysis Kit (2G) is used to quantitate Vero DNA residues of different fragment sizes for various stages of biopharmaceutical products, from in-process samples to final products. This kit utilizes real-time PCR technology to perform rapid and specific quantitation of residual Vero DNA fragments in samples. It is designed to amplify four different fragments (85bp, 134bp, 229bp, 552bp) for the accurate determination of their size distribution with the LOD level of femtograms. For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).
Introduction
| Components | Shipping Condition | Unit Size | Detection Method |
| qPCR Reaction Buffer | -20℃, protect from light | 4x100 Reactions | Probe-based qPCR |
| Vero Primer&Probe MIX-85 | |||
| Vero Primer&Probe MIX-134 | |||
| Vero Primer&Probe MIX-229 | |||
| Vero Primer&Probe MIX-552 | |||
| IPC MIX | |||
| DNA Dilution Buffer (DDB) | -20℃ | ||
| Vero DNA Control |
Specification
| Range | 3x10-2-3x102 pg/μL, R2 ≥0.990, E= 83.3%-110% |
| Accuracy | Recovery = 71.1%-99.4% , CV<40% |
| LOQ | 0.03 pg/μL |
| LOD | 0.006 pg/μL |
| Precision | 5.5%-24.2% (<40%) |
| Robustness | Freeze-thaw stability over 5 cycles |
| Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments |
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When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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