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For Research Use Only (RUO)
Description
SHENTEK® Residual SV40LTA & E1A DNA Quantitation Kit (2G) is used to quantitate residual SV40LTA & E1A host cell DNA, such as HEK293T cell. This sv40 large t antigen kit uses duplex real-time PCR technique to performs rapid, specific, and reliable quantitation assay at the level of 101 copies/μL. For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191)
Introduction
| Components | Shipping Condition | Unit Size | Detection Method |
| SV40LTA & E1A linear DNA Control | -20℃ | 100 Reactions | Probe-basedq PCR |
| SV40LTA & E1A non-linear DNA Control | |||
| qPCR Reaction Buffer | -20℃, protect from light | ||
| SV40LTA & E1A Primer & Probe MIX | |||
| IPC MIX | |||
| DNA Dilution Buffer (DDB) | -20℃ |
Specification
| Range | SV40LTA: 4.67x101-4.67x106 pg/μL, R2=0.99974, E=97.5% |
| E1A: 4.97x101-4.97x106 pg/μL, R2=0.99946, E=98.3% | |
| Accuracy |
SV40LTA: Recovery = 105.2%-110.1%, CV<30% |
| E1A: Recovery = 115.8%-124.5%, CV<30% | |
| LOQ |
SV40LTA: 9.34 copies/μL |
| E1A: 49.7 copies/μL | |
|
LOD |
SV40LTA: 0.934 copies/μL |
| E1A: 0.497 copies/μL | |
|
Precision |
SV40LTA: 1.7%-9.0% (<30%) |
| E1A: 1.9%-9.4%(<30%) | |
|
Robustness |
Freeze-thaw stability over 5 cycles |
| Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments |
Documents
Related Products
SHENTEK™ Residual Host Cell DNA Sample Preparation Kit
Research Use Only
For Research Use Only (RUO) – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.
Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.

