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Description
HENTEK® Residual Human DNA Quantitation Kit (2G) is used to quantitate residual Human host cell DNA of biopharmaceutical products in different stages, from in-process samples to final products. This kit utilizes duplex quantitative PCR (qPCR) technique to performs a rapid, specific, and reliable quantitation assay at femtogram (fg) level. Internal Positive Control (IPC) is included in the Human Primer&Probe MIX to evaluate the performance of each PCR reaction. For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).
Overview
Components | Shipping Condition | Unit Size | Detection Method |
Human DNA Control | -20℃ | 100 Reactions | Probe-based qPCR |
qPCR Master MIX | -20℃, protect from light | ||
Human Primer&Probe MIX (Incl IPC) | |||
DNA Dilution Buffer (DDB) | -20℃ |
Specification
Range | 3x10-2-3x102 pg/μL, R2=0.99940, E=95.6% |
Accuracy | Recovery = 65.9% - 97.7%, CV<30% |
LOQ | 0.03 pg/μL |
Precision | 5.1%-16.1% (<30%) |
Robustness | Freeze-thaw stability over 5 cycles |
Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments |
Documents
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When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.