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Description
SHENTEK® Residual E1B DNA Quantitation Kit is used to quantitate residual E1B DNA from host cell, such as HEK293 cell and its derivative cell lines (e.g. 293T and 293F, etc.), in different stages of biopharmaceutical products, from in-process samples to final products. This kit utilizes fluorescent quantitative PCR technique to perform rapid, specific, and reliable quantitation assay for residual E1B DNA. IPC (Internal Positive Control) is included in the kit to evaluate the performance of each PCR reaction. The kit provides E1B linear DNA Control for customers to detect of linearized samples. For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).
Overview
| Components | Shipping Condition | Unit Size | Detection Method |
| E1B linear DNA Control | -20℃ | 100 Reactions | Probe-based qPCR |
| qPCR Reaction Buffer | -20℃, protect from light | ||
| E1B Primer&Probe MIX | |||
| IPC MIX | |||
| DNA Dilution Buffer (DDB) | -20℃ |
Specification
| Range | 4.34x101-4.34x106 pg/μL, R2=0.998, E=96.5% |
| Accuracy | Recovery = 92.4%-102.4%, CV<30% |
| LOQ | 8.68 copies/μL |
| LOD | 0.868 copies /μL |
| Precision | 6.0%-19.4% (<30%) |
| Robustness | Freeze-thaw stability over 5 cycles |
| Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments |
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When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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