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Introduction
| Components | Shipping Condition | Unit Size | Product Line | Detection Method |
| E.coli RNA Control | -20℃ | 100 Reactions | SHENTEK™ | Probe-based qPCR |
| One Step qPCR Buffer | -20℃, protect from light | |||
| One Step Enzyme MIX | -20℃, protect from light | |||
| E.coli RNA Primer&Probe MIX (Incl IPC) | -20℃, protect from light | |||
| RNase-Free H2O | -20℃ |
Overview
SHENTEK™ Residual E. coli RNA Quantitation Kit (2G) is used for quantitation of host cell RNA from E. coli in a variety of biopharmaceutical products. This kit employs duplex reverse transcription quantitative PCR technique to performs a rapid, specific, and reliable quantitative detection of residual E. coli RNA .The target gene (FAM) can be determined at the femtogram (fg) level, whereas IPC-Internal Positive Control (VIC) is also included in the E. coli RNA Primer&Probe MIX to evaluate the performance of each PCR reaction.
Specification
| Range | 2x10-2-2x102pg/μL, R2 ≥0.990，E=83.3%~110% |
| Accuracy | 64.1%-97.8%, CV<30% |
| LOQ | 0.002 pg/μL |
| Precision | 10.7%-13.1% (<30%) |
| Robustness | Freeze-thaw stability over 5 cycles |
| Instrument suitability not limited to Thermo, Biorad, HZSKBio qPCR equipments |
Related Products
SHENTEK™ Residual Host Cell RNA Sample Preparation Kit
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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