Description

SHENTEK® CAR/TCR Transgene Copy Number Quantitation Kit is designed to quantify the copy number of target genes in human cell products derived from common HIV-1 lentiviral vectors, such as CAR or TCR genes integrated in CAR-T and TCR-T cell genomes.

CAR/TCR Transgene Copy Number Quantitation Kit utilizes multiplex qPCR technique to detect DNA sequences related to the integration or expression function on the transfer plasmid and Single Copy Gene (SCG) in human cells. Average copies of target gene per cell can be calculated in the samples, such as CAR or TCR gene copy numbers. This kit is rapid, specific, reliable, and universal for lentiviral vector applications. The reference standard in this kit is produced by our company.

Overview

Components	Shipping Condition	Unit Size	Detection Method
qPCR Reaction Buffer	-20℃, protect from light	100 Reactions	Probe-based qPCR
CAR/TCR Primer&Probe MIX
IPC MIX
DNA Dilution Buffer (DDB)
CAR/TCR Control	-20℃

Specification

Range	CAR/TCR: 2.33×10¹-2.33×10⁷ copies/μL, R²= 0.999829，E=94.9%
Range	SCG: 2.33×10²-2.33×10⁷, R²= 0.999564, E=94.8%
Accuracy	Recovery = 104.7%-137.6%, CV<20%
LOQ	CAR/TCR: 2.33×10¹ copies/μL
LOQ	SCG: 5.285×10¹ copies/μL
LOD	2.33 copies/μL
Precision	5.9%-9.1% (<15%)
Robustness	Freeze-thaw stability over 5 cycles
Robustness	Instrument suitability not limited to Thermo, Biorad, Bioer, SHENTEK qPCR equipments

Documents

User Guide

Related Products

SHENTEK™ Residual Host Cell DNA Sample Preparation Kit

CAR/TCR Transgene Copy Number Quantitation Kit SHENTEK®