For Research Use Only (RUO)

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Description

The pcr amplification kits are used for quantitation of residual E. coli host cell DNA in a variety of biopharmaceutical products.

SHENTEK® Residual E.coli DNA Quantitation Kit is used for quantitation of residual E. coli host cell DNA in a variety of biopharmaceutical products. This kit uses quantitative PCR to perform rapid and specific quantitation of residual DNA at the femtogram level. The assay is accurate and reliable across a broad range of sample types, from in-process samples to final products. The kit comes with E. coli Control DNA, which is traceable to a primary DNA reference standard.

For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. SK030203D100)

Introduction 

Components Shipping Condition Unit Size Detection Method
E. coli DNA Control -20℃ 100 Reactions Probe-based qPCR
E. coli qPCR MIX -20℃, protect from light
DNA Dilution Buffer (DDB) -20℃

 

Specification

Range 3x10-2-3x102 pg/μL, R2=0.99968, E=97.5%
Accuracy Recovery = 81.2%-107.4% , CV<30%
LOQ 0.03 pg/μL
LOD 0.003 pg/μL
Precision 2.8%-17.9% (<30%)
Robustness Freeze-thaw stability over 5 cycles
Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments

 

Documents

User Guide

Related Products

Residual Host Cell DNA Sample Preparation Kit - SHENTEK® 

Research Use Only

For Research Use Only (RUO) – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.

Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

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