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Sku: 1104197
Categories: Kit

Introduction

Components Shipping Condition Unit Size Product Line Detection Method
E. coli DNA Control -20℃ 100 Reactions SHENTEK™ Probe-based qPCR
qPCR Master MIX -20℃ - - -
DNA Dilution Buffer (DDB) -20℃ - - -
E. coli Primer&Probe MIX (Incl IPC) -20℃, protect from light - - -

 

 

Overview

SHENTEK™ Residual E. coli DNA Quantitation Kit is used to quantitate residual E. coli host cell DNA in different stages of biopharmaceutical products, from in-process samples to final products. This kit uses duplex real-time PCR technology to perform rapid, specific, and reliable quantitation assay at the fg level. IPC(Internal Positive Control) is included in the E. coli Primer&Probe MIX to evaluate the performance of each PCR reaction. For extraction information, please refer to the SHENTEKTM Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191-E).

 

Specification

Range 3.00E+02 to 3.00E-02 pg/μL, R2=0.99968, AE=97.5%
Accuracy Recovery = 50%-150%, CV<30%
LOQ 0.03 pg/μL
LOD 0.003 pg/μL
Precision CV <30%
Robustness

Freeze-thaw stability over 5 cycles

Instrument suitability not limited to Thermo, Biorad, HZSKBio qPCR equipments

 

 

Related Products

Residual Host Cell DNA Sample Preparation Kit - SHENTEK® 

 

Documents

User Guide

Assay Validation Summary

Assay Validation Report (on Request)

Flyer

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

Terms and Conditions

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