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Sku: TL-650-0050
Recombinant Human TPO Protein
Description
Recombinant Human Thrombopoietin (TPO) is a functional protein transiently expressed in HEK-293 cells with a C-terminal 6×His tag.
By retaining the N-terminal 1–174 amino acid residues of TPO (the EPO-homologous domain essential for bioactivity) and removing the non-essential C-terminal glycosylated region, its molecular weight is optimized to 18–23 kDa.
This protein specifically binds to the c-Mpl receptor and activates the JAK/STAT signaling pathway, playing a central role in hematopoietic stem cell culture and cell therapy applications.
It drives directed differentiation of hematopoietic stem/progenitor cells toward the megakaryocytic lineage, efficiently promoting in vitro expansion and maturation of megakaryocytes, as well as platelet production.
This provides critical support for establishing functional in vitro platelet production platforms, developing thrombocytopenia therapeutics, and advancing stem cell-based regenerative therapies.
Specifications
| Parameter | Specification | Results |
|---|---|---|
| Appearance | Freeze-dried preparation, the form should be white loose body | Compliance |
| Identity | The electrophoretic bands should be consistent with the molecular weight of the product | Compliance |
| Visible Particle | Should be no visible particle | Compliance |
| Moisture | ≤4.0% | 2.8% |
| pH | 6.5~7.5 | 7.3 |
| Purity | >90.0% | Compliance |
| Endotoxin | <0.1EU/μg | <0.1EU/μg |
| Mycoplasma | Should be negative | Negative |
| Sterility | No growth | No growth |
| Biological Activity | Cell proliferation assay performed with Mo7e cells demonstrated specific activity ≥ 5.0 × 106 IU/mg | 3.13 × 107 IU/mg |
Features
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✔
HEK293-Expressed Truncated TPO (Ser22–Leu195)
Recombinant human TPO expressed in HEK293 cells, corresponding to Ser22–Leu195 with Pro157Asn and Arg164Asn substitutions, retaining the EPO-homologous bioactive domain and optimized molecular weight (~19.5 kDa), with a C-terminal 6×His tag.
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✔
High Purity and Controlled Quality Attributes
Purity >90% by SDS-PAGE, electrophoretic identity consistent with expected molecular weight, no visible particles, moisture 2.8%, pH 7.3 within 6.5–7.5 specification.
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✔
Low Endotoxin and Sterility Assurance
Endotoxin <0.1 EU per µg (LAL method), mycoplasma negative, and sterility confirmed with no growth.
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✔
Validated Mo7e Proliferation Activity
Biological activity measured by Mo7e cell proliferation assay, with specific activity ≥5.0 × 106 IU/mg (measured 3.13 × 107 IU/mg).
Applications
HSPC Culture Support
Binds to c-Mpl receptor and activates JAK/STAT signaling to support hematopoietic stem/progenitor cell expansion.
Megakaryocytic Differentiation
Drives directed differentiation of hematopoietic stem/progenitor cells toward the megakaryocyte lineage in vitro.
Megakaryocyte Maturation Studies
Promotes in vitro expansion and maturation of megakaryocytes for platelet generation studies.
In Vitro Platelet Platforms
Supports development of functional in vitro platelet production systems and stem cell–based regenerative research.
Data Figures
Stability & Storage
- Lyophilized powder: Stable for 12 months at −80 °C or 6 months at −20 °C when stored in the original sealed container under desiccant.
- Reconstitution: Dissolve in sterile water for injection, 0.9% NaCl, or PBS (pH7.4), maintaining a final concentration ≥100 µg/mL to prevent adsorption.
- Handling: Aliquot to avoid repeated freeze–thaw cycles.
Cautions
• Research Use Only: This product is not intended for diagnostic or therapeutic use.
• Handling: Prepare solutions using sterile technique. Avoid microbial contamination to maintain activity.
• Storage: Follow recommended storage conditions. Repeated freeze–thaw cycles may reduce performance.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
