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$126.00
Sku: TL-504-0010
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by June 09, 2026
Description
Recombinant Human Stem Cell Factor (SCF) is a multifunctional cytokine produced by fibroblasts and endothelial cells that binds the c-Kit receptor, existing in soluble (~18.5 kDa dimer) and transmembrane forms via alternative splicing.
During development, it regulates hematopoiesis (fetal liver/bone marrow), germ cell and neural development, and melanocyte localization. In adulthood, it maintains hematopoietic stem cell (HSC) homeostasis by governing survival, proliferation, differentiation, and mobilization within the bone marrow niche, while also stimulating mast cell growth.
In vitro, SCF potently synergizes with cytokines like G-CSF, GM-CSF, and IL-3 to stimulate hematopoietic progenitor proliferation and differentiation. In vivo, combined with G-CSF, it clinically enhances peripheral blood CD34+ cell yields by 2–3 fold versus G-CSF alone.
This product is GMP manufactured in mammalian cells with strict control of residuals (host protein, nucleic acid) and pathogens, ensuring full traceability.
Specifications
| Parameter | Specification |
|---|---|
| Synonyms | C-kit ligand; Mast cell growth factor (MGF); Stem cell factor |
| Accession | UniProt P21583-1 |
| Expressed Region | Glu26-Ala189 |
| Tag | C-terminal 6×His-tag |
| Expression System | HEK293 cells |
| Predicted Molecular Weight | 19.3 kDa |
| Purity | > 90% as determined by SDS-PAGE |
| Endotoxin | < 0.1 EU per 1 µg of protein (LAL method) |
| Activity | Measured in a cell proliferation assay using Mo7e cells, corresponding to a specific activity of ≥ 2.0 × 105 IU/mg |
| Form | Lyophilized from sterile PBS (pH 7.4), typically supplemented with 6% mannitol as a protectant |
Features
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✔
HEK293-Expressed SCF (Glu26–Ala189)
Recombinant human SCF corresponding to Glu26–Ala189, expressed in HEK293 cells with a C-terminal 6×His-tag; predicted molecular weight 19.3 kDa.
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✔
High Purity with Defined Identity
Purity > 90% as determined by SDS-PAGE, supporting reliable performance in cell-based workflows.
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✔
Low Endotoxin for Sensitive Cell Culture
Endotoxin < 0.1 EU per 1 µg of protein (LAL method), helping reduce background responses in hematopoietic cultures.
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✔
Validated Mo7e Proliferation Bioactivity
Activity measured in a Mo7e cell proliferation assay, corresponding to a specific activity of ≥ 2.0 × 105 IU/mg.
Applications
HSC Homeostasis Support
Binds the c-Kit receptor to support HSC survival, proliferation, differentiation, and mobilization in vitro.
Progenitor Cell Expansion
Used to stimulate hematopoietic progenitor proliferation and differentiation, including synergistic use with other cytokines.
Mast Cell Growth Studies
Supports mast cell growth and related immunology research workflows.
CD34+ Mobilization Research
Applied in studies evaluating increased peripheral blood CD34+ yields in combination settings such as G-CSF.
Data Figures
Stability & Storage
TL-504
- Lyophilized powder: Stable for 12 months at -80°C or 6 months at -20°C when stored in the original sealed container under desiccant.
- Reconstitution: Dissolve contents in 200 µL sterile water for injection, 0.9% NaCl, or PBS (pH7.4), maintaining a final concentration about 50 µg/mL.
- Handling: Aliquot to avoid repeated freeze-thaw cycles.
GMP-TL504
- Lyophilized powder: Stable for 12 months at -80°C or 6 months at -20°C when stored in the original sealed container under desiccant.
- Reconstitution: Dissolve in sterile water for injection, 0.9% NaCl, or PBS (pH7.4), maintaining a final concentration ≥ 100 µg/mL to prevent adsorption.
- Handling: Aliquot to avoid repeated freeze-thaw cycles.
Cautions
• Research Use Only: This product is not intended for diagnostic or therapeutic use.
• Handling: Prepare solutions using sterile technique. Avoid microbial contamination to maintain activity.
• Storage: Follow recommended storage conditions. Repeated freeze–thaw cycles may reduce performance.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
